Discrepancies between flow cytometric analysis and [3H]thymidine incorporation in stimulated lymphocytes

M. Sasvári, Gábor Szabó, M. Staub, Tatjana Spasokukotskaja, Ferenc Antoni

Research output: Contribution to journalArticle

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Abstract

The DNA synthesis system of freshly isolated tonsillar lymphocytes and those stimulated by phytohaemagglutinin were compared by different methods. Both cell populations had high DNA polymerase α and thymidine kinase activities, as well as a high rate of incorporation of [3H]thymidine into DNA. However, the two cell populations differed when their DNA distributions were compared by flow cytometry. Freshly isolated cells contained many less (6%) cells in S phase than were found in phytohaemagglutinin-stimulated lymphocytes (18%) as detected by flow cytometry. The labelling of different subpopulations of lymphocytes was studied by sorting them electrically with a fluorescence-activated cell sorter. Analysis of the radioactivity of [3H]thymidine pulse-labelled cells, sorted according to their DNA content, showed that cells in the G1 peak of DNA distribution had a significant amount of incorporated [3H]thymidine. Sorting of cells according to their size (i.e., by light scattering) revealed that only large cells were labelled with [3H]thymidine.

Original languageEnglish
Pages (from-to)452-457
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume762
Issue number3
DOIs
Publication statusPublished - Jun 2 1983

Fingerprint

Thymidine
Lymphocytes
DNA
Phytohemagglutinins
Flow Cytometry
Polynucleotide 5'-Hydroxyl-Kinase
Thymidine Kinase
Lymphocyte Subsets
DNA-Directed DNA Polymerase
S Phase
Radioactivity
Population
Fluorescence
Light

Keywords

  • (Lymphocyte)
  • Flow cytometry
  • Phytohemagglutinin stimulation
  • Thymidine incorporation

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Discrepancies between flow cytometric analysis and [3H]thymidine incorporation in stimulated lymphocytes. / Sasvári, M.; Szabó, Gábor; Staub, M.; Spasokukotskaja, Tatjana; Antoni, Ferenc.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 762, No. 3, 02.06.1983, p. 452-457.

Research output: Contribution to journalArticle

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