Disassembly of chromatin into ≅50 kb units by detergent

G. Szabó, F. Boldog, N. Wikonkál

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Nuclei isolated from higher eukaryotic cell lines were directly analyzed by field inversion gel electrophoresis. Brief incubation of nuclei with ionic detergents yielded a single band between 50-100 kb. The apparent fragment size decreased to ≅50 kb after proteinase digestion. The latter treatment alone induced less regular, ≦50 kb fragmentation. DNA extracted from detergent and proteinase-treated nuclei also appeared in a band of about 40 kb. Embedding into agarose plugs did not protect nuclei, as opposed to cells, from detergent-induced fragmentation. The phenomenon is strikingly analogous to the double-strand DNA cleavage reactions mediated by topoisomerase II. Our data are compatible with any of the following interpretations: 1.) regularly spaced protein bridges, probably involving topoisomerase II, maintain or control continuity of chromosomal DNA in certain states of higher eukaryotic cells. 2.) The DNA might become accessible to a putative endonuclease at regularly spaced sites upon detergent treatment of isolated nuclei.

Original languageEnglish
Pages (from-to)706-712
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume169
Issue number2
DOIs
Publication statusPublished - Jun 15 1990

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Chromatin Assembly and Disassembly
Detergents
Chromatin
Type II DNA Topoisomerase
DNA
Eukaryotic Cells
Peptide Hydrolases
DNA Cleavage
Endonucleases
Pulsed Field Gel Electrophoresis
Electrophoresis
Sepharose
Digestion
Gels
Cells
Cell Line
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Disassembly of chromatin into ≅50 kb units by detergent. / Szabó, G.; Boldog, F.; Wikonkál, N.

In: Biochemical and Biophysical Research Communications, Vol. 169, No. 2, 15.06.1990, p. 706-712.

Research output: Contribution to journalArticle

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