Direct visualization of surface-adsorbed single fluorescently labeled titin molecules

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The properties of titin, a giant, structurally and mechanically complex filamentous protein were explored here by using single-molecule techniques. Fluorescently labeled titin molecules deposited on a glass substrate surface were directly visualized by using confocal microscopy. The molecules appeared as bright particles with a spatially distributed Gaussian fluorescence intensity profile. The physical dimensions of the space occupied by the 1-μm-long molecule on the surface are below the 0.25 μm resolution limit of the instrument used here, thereby directly demonstrating the flexible nature of the titin filament. The kinetics of titin's surface adsorption revealed that the process is governed by diffusion and the molecules are irreversibly bound to the substrate surface. From the kinetics data the molecule's translational diffusion constant was calculated.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalSingle Molecules
Volume2
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

ASJC Scopus subject areas

  • Chemistry(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

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