Direct tandem mass spectrometric analysis of amino acids in dried blood spots without chemical derivatization for neonatal screening

Kornél Nagy, Zoltán Takáts, Ferenc Pollreisz, T. Szabó, K. Vékey

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Neonatal screening performed by electrospray tandem mass spectrometry is a powerful technique in clinical diagnostics. In the present paper an alternative to the widely accepted method involving butylation has been developed. In the new method butylation is not required, and multiple reaction monitoring (MRM) was used instead of constant neutral loss scanning. The method was optimized for detection of 23 L-amino acids in their native form. Quantitation was based on isotope-labeled internal standards, calibration curves were linear from 0 to 500 μmol/L, and detection limits were in the range 2-42 μmol/L. The utility of the present technique is illustrated in the case of one neonate suffering from citrullinaemia.

Original languageEnglish
Pages (from-to)983-990
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume17
Issue number9
Publication statusPublished - 2003

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Isotopes
Mass spectrometry
Screening
Blood
Calibration
Scanning
Amino Acids
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Direct tandem mass spectrometric analysis of amino acids in dried blood spots without chemical derivatization for neonatal screening. / Nagy, Kornél; Takáts, Zoltán; Pollreisz, Ferenc; Szabó, T.; Vékey, K.

In: Rapid Communications in Mass Spectrometry, Vol. 17, No. 9, 2003, p. 983-990.

Research output: Contribution to journalArticle

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