Direct observation of multistep energy transfer in LHCII with fifth-order 3D electronic spectroscopy

Zhengyang Zhang, P. Lambrev, Kym L. Wells, G. Garab, Howe Siang Tan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

During photosynthesis, sunlight is efficiently captured by light-harvesting complexes, and the excitation energy is then funneled towards the reaction centre. These photosynthetic excitation energy transfer (EET) pathways are complex and proceed in a multistep fashion. Ultrafast two-dimensional electronic spectroscopy (2DES) is an important tool to study EET processes in photosynthetic complexes. However, the multistep EET processes can only be indirectly inferred by correlating different cross peaks from a series of 2DES spectra. Here we directly observe multistep EET processes in LHCII using ultrafast fifth-order three-dimensional electronic spectroscopy (3DES). We measure cross peaks in 3DES spectra of LHCII that directly indicate energy transfer from excitons in the chlorophyll b (Chl b) manifold to the low-energy level chlorophyll a (Chl a) via mid-level Chl a energy states. This new spectroscopic technique allows scientists to move a step towards mapping the complete complex EET processes in photosynthetic systems.

Original languageEnglish
Article number7914
JournalNature Communications
Volume6
DOIs
Publication statusPublished - Jul 31 2015

Fingerprint

Excitation energy
Energy Transfer
Energy transfer
Spectrum Analysis
energy transfer
Observation
Spectroscopy
chlorophylls
electronics
spectroscopy
excitation
Electron energy levels
Photosynthetic Reaction Center Complex Proteins
photosynthesis
Photosynthesis
Sunlight
sunlight
energy levels
excitons
Light

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Direct observation of multistep energy transfer in LHCII with fifth-order 3D electronic spectroscopy. / Zhang, Zhengyang; Lambrev, P.; Wells, Kym L.; Garab, G.; Tan, Howe Siang.

In: Nature Communications, Vol. 6, 7914, 31.07.2015.

Research output: Contribution to journalArticle

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