Direct measurement of torque in an optical trap and its application to double-strand DNA

László Oroszi, Péter Galajda, Huba Kirei, Sándor Bottka, Pál Ormos

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We present a method that offers the possibility to directly apply and measure torque on particles in an optical trap. It can be used to rotationally manipulate biopolymers attached to appropriate particles. A flat object is trapped and oriented in the focus of a linearly polarized laser light. The direction and power of the orientational trap are controlled by the polarization state of the light. As a demonstration of the capabilities of the method, we examined the torsional stiffness of dsDNA (λ-DNA) in its linear torsional regime by directly measuring the torque generated by the molecule.

Original languageEnglish
Article number058301
JournalPhysical review letters
Volume97
Issue number5
DOIs
Publication statusPublished - Aug 7 2006

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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