Direct interaction of flagellin termini essential for polymorphic ability of flagellar filament

Yuko Mimori-Kiyosue, F. Vonderviszt, Ichiro Yamashita, Yoshinori Fujiyoshi, Keiichi Namba

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We report the structures of flagellar filaments reconstituted from various flagellins with small terminal truncations. Flagellins from Salmonella typhimurium strains SJW1103 (wild type), SJW1660, and SJW1655 were used, which form a left-handed supercoil, the L- and R-type straight forms, respectively. Structure analyses were done by electron cryomicroscopy and helical image reconstruction with a help of x-ray fiber diffraction for determining precise helical symmetries. Truncation of either terminal region, irrespective of the original flagellin species, results in a straight filament having a helical symmetry distinct either from the L- or R-type. This filament structure is named Lt-type. Although the local subunit parking is similar in all three types, a close comparison shows that the Lt-type packing is almost identical to the R-type but distinct from the L-type, which demonstrates the strong two-state preference of the subunit interactions. The structure clearly suggests that both termini are located in the inner tube of the concentric double-tubular structure of the filament core, and their proper interaction is responsible for the correct folding of fairly large terminal regions that form the inner tube. The double tubular structure appears to be essential for the polymorphic ability of flagellar filaments, which is required for the swimming-tumbling of bacterial taxis.

Original languageEnglish
Pages (from-to)15108-15113
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number26
DOIs
Publication statusPublished - Dec 24 1996

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Flagellin
Cryoelectron Microscopy
Computer-Assisted Image Processing
Salmonella typhimurium
X-Rays

ASJC Scopus subject areas

  • Genetics
  • General

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Direct interaction of flagellin termini essential for polymorphic ability of flagellar filament. / Mimori-Kiyosue, Yuko; Vonderviszt, F.; Yamashita, Ichiro; Fujiyoshi, Yoshinori; Namba, Keiichi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 26, 24.12.1996, p. 15108-15113.

Research output: Contribution to journalArticle

Mimori-Kiyosue, Yuko ; Vonderviszt, F. ; Yamashita, Ichiro ; Fujiyoshi, Yoshinori ; Namba, Keiichi. / Direct interaction of flagellin termini essential for polymorphic ability of flagellar filament. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 26. pp. 15108-15113.
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