Direct evaluation of effects of fatty-acid unsaturation on the thermal properties of photosynthetic activities, as studied by mutation and transformation of synechocystis PCC6803

Z. Gombos, Hajime Wada, Norio Murata

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Abstract

Glycerolipids of thylakoid membranes isolated from the cyanobacterium Synechocystis PCC6803 contained high levels of dienoic and trienoic C18 fatty acids, in addition to saturated C16 and monoenoic C18 fatty acids. A mutant (Fadl2) of this cyanobacterium was defective in the desaturation of C18 fatty acids at the δ12 position, and its thylakoid membranes lacked trienoic acids and contained a very reduced level of dienoic acids. A derivative strain of Fadl2 (Fadl2/desA), which had been transformed with a gene for desaturation at the δ12 position, fully recovered the ability to desaturate the fatty acids in the glycerolipids of thylakoid membranes. The thermal properties of the photosynthetic activities of the mutant and the transformant were compared with those of the wild-type strain. Despite great diversity in the extent of unsaturation of fatty acids between the wild-type, Fadl2, and Fad12/desA strains, no significant differences were found either in the temperature dependence of photosynthesis or in the heat stability of photosynthetic, photosystem II and photosystem I activities. These results demonstrate that the trienoic fatty acids and, probably, the dienoic acids of the lipids in the thylakoid membrane do not affect the thermal properties of the above-mentioned activities of photosynthesis.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalPlant and Cell Physiology
Volume32
Issue number2
Publication statusPublished - Mar 1991

Fingerprint

Synechocystis
Thermal Properties
Fatty Acids
thermal properties
Fatty acids
Thylakoid Membrane
mutation
Mutation
Thylakoids
fatty acid
Thermodynamic properties
thylakoids
Hot Temperature
fatty acids
Evaluation
membrane
Membranes
Cyanobacteria
Photosynthesis
acids

Keywords

  • Fatty acid desaturation
  • Mutation
  • Photosynthesis
  • Synechocystis PCC6803
  • Thermal properties
  • Transformation

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

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title = "Direct evaluation of effects of fatty-acid unsaturation on the thermal properties of photosynthetic activities, as studied by mutation and transformation of synechocystis PCC6803",
abstract = "Glycerolipids of thylakoid membranes isolated from the cyanobacterium Synechocystis PCC6803 contained high levels of dienoic and trienoic C18 fatty acids, in addition to saturated C16 and monoenoic C18 fatty acids. A mutant (Fadl2) of this cyanobacterium was defective in the desaturation of C18 fatty acids at the δ12 position, and its thylakoid membranes lacked trienoic acids and contained a very reduced level of dienoic acids. A derivative strain of Fadl2 (Fadl2/desA), which had been transformed with a gene for desaturation at the δ12 position, fully recovered the ability to desaturate the fatty acids in the glycerolipids of thylakoid membranes. The thermal properties of the photosynthetic activities of the mutant and the transformant were compared with those of the wild-type strain. Despite great diversity in the extent of unsaturation of fatty acids between the wild-type, Fadl2, and Fad12/desA strains, no significant differences were found either in the temperature dependence of photosynthesis or in the heat stability of photosynthetic, photosystem II and photosystem I activities. These results demonstrate that the trienoic fatty acids and, probably, the dienoic acids of the lipids in the thylakoid membrane do not affect the thermal properties of the above-mentioned activities of photosynthesis.",
keywords = "Fatty acid desaturation, Mutation, Photosynthesis, Synechocystis PCC6803, Thermal properties, Transformation",
author = "Z. Gombos and Hajime Wada and Norio Murata",
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T1 - Direct evaluation of effects of fatty-acid unsaturation on the thermal properties of photosynthetic activities, as studied by mutation and transformation of synechocystis PCC6803

AU - Gombos, Z.

AU - Wada, Hajime

AU - Murata, Norio

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N2 - Glycerolipids of thylakoid membranes isolated from the cyanobacterium Synechocystis PCC6803 contained high levels of dienoic and trienoic C18 fatty acids, in addition to saturated C16 and monoenoic C18 fatty acids. A mutant (Fadl2) of this cyanobacterium was defective in the desaturation of C18 fatty acids at the δ12 position, and its thylakoid membranes lacked trienoic acids and contained a very reduced level of dienoic acids. A derivative strain of Fadl2 (Fadl2/desA), which had been transformed with a gene for desaturation at the δ12 position, fully recovered the ability to desaturate the fatty acids in the glycerolipids of thylakoid membranes. The thermal properties of the photosynthetic activities of the mutant and the transformant were compared with those of the wild-type strain. Despite great diversity in the extent of unsaturation of fatty acids between the wild-type, Fadl2, and Fad12/desA strains, no significant differences were found either in the temperature dependence of photosynthesis or in the heat stability of photosynthetic, photosystem II and photosystem I activities. These results demonstrate that the trienoic fatty acids and, probably, the dienoic acids of the lipids in the thylakoid membrane do not affect the thermal properties of the above-mentioned activities of photosynthesis.

AB - Glycerolipids of thylakoid membranes isolated from the cyanobacterium Synechocystis PCC6803 contained high levels of dienoic and trienoic C18 fatty acids, in addition to saturated C16 and monoenoic C18 fatty acids. A mutant (Fadl2) of this cyanobacterium was defective in the desaturation of C18 fatty acids at the δ12 position, and its thylakoid membranes lacked trienoic acids and contained a very reduced level of dienoic acids. A derivative strain of Fadl2 (Fadl2/desA), which had been transformed with a gene for desaturation at the δ12 position, fully recovered the ability to desaturate the fatty acids in the glycerolipids of thylakoid membranes. The thermal properties of the photosynthetic activities of the mutant and the transformant were compared with those of the wild-type strain. Despite great diversity in the extent of unsaturation of fatty acids between the wild-type, Fadl2, and Fad12/desA strains, no significant differences were found either in the temperature dependence of photosynthesis or in the heat stability of photosynthetic, photosystem II and photosystem I activities. These results demonstrate that the trienoic fatty acids and, probably, the dienoic acids of the lipids in the thylakoid membrane do not affect the thermal properties of the above-mentioned activities of photosynthesis.

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