Direct effect of colchicine on the microspore embryogenesis to produce dihaploid plants in wheat (Triticum aestivum L.)

B. Barnabás, P. L. Pfahler, G. Kovács

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93 Citations (Scopus)

Abstract

In the present experiment, different chromosome reduplication techniques were applied to microspore-originated Triticum aestivum L. (cv 'Ciano') haploids. In addition to the conventional treatment (whole plant exposure to colchicine solution), spontaneously redoubled haploids were also examined. As an experimental treatment, different concentrations (0.01, 0.02, 0.04%) of colchicine were added directly to the induction media. Colchicine did not affect the anther response or the plant regeneration capacity. The success and stability of genome redoubling was estimated on the basis of the fertility of the regenerated (R0) plants and their progeny (R1). Chromosome doubling produced by colchicine before the first microspore mitosis was significantly more efficient than the conventionally used techniques.

Original languageEnglish
Pages (from-to)675-678
Number of pages4
JournalTheoretical And Applied Genetics
Volume81
Issue number5
DOIs
Publication statusPublished - May 1991

Fingerprint

dihaploidy
colchicine
Colchicine
microspores
Triticum
Embryonic Development
Triticum aestivum
embryogenesis
wheat
Haploidy
haploidy
Chromosomes
chromosomes
Genomic Instability
Mitosis
anthers
mitosis
Fertility
Regeneration
genome

Keywords

  • Anther culture
  • Chromosome duplication
  • Colchicine
  • Fertility of regenerants
  • Wheat

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

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title = "Direct effect of colchicine on the microspore embryogenesis to produce dihaploid plants in wheat (Triticum aestivum L.)",
abstract = "In the present experiment, different chromosome reduplication techniques were applied to microspore-originated Triticum aestivum L. (cv 'Ciano') haploids. In addition to the conventional treatment (whole plant exposure to colchicine solution), spontaneously redoubled haploids were also examined. As an experimental treatment, different concentrations (0.01, 0.02, 0.04{\%}) of colchicine were added directly to the induction media. Colchicine did not affect the anther response or the plant regeneration capacity. The success and stability of genome redoubling was estimated on the basis of the fertility of the regenerated (R0) plants and their progeny (R1). Chromosome doubling produced by colchicine before the first microspore mitosis was significantly more efficient than the conventionally used techniques.",
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AU - Pfahler, P. L.

AU - Kovács, G.

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