The determination of the vibrational density of states of a protein (dihydrofolate reductase) on binding a ligand (methotrexate) was determined using inelastic neutron scattering. Inelastic neutron scattering, in which the dynamic structure S (q,ω) was measured as a function of the scattering wavevector q and energy transfer hω has been used to determine the vibrational density of the states for several proteins. The study suggests that the direct access to the vibrational density of states provides the study of vibrational thermodynamic changes in many biomolecular association processes. The free-energy change, which was directly determined by the density of states change, was found to contribute significantly to the binding equilibrium.
ASJC Scopus subject areas
- Physics and Astronomy(all)