Direct cellular effects of some mediators, hormones and growth factor-like agents on denervated (isolated) rat gastric mucosal cells

B. Bódis, O. Karádi, L. Nagy, Cs Dohoczky, M. Kolega, Gy Mózsik

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The brain-gut axis has an important role in the mechanism of gastric cytoprotection in vivo. The aim of this study was to evaluate the in vitro effect of protective agents without any central and peripheral innervation. A mixed population of rat gastric mucosal cells was isolated by the method of Nagy et al (Gastroenterology (1994) 77, 433-443). Cells were incubated for 60 min with cytoprotective drugs such as prostacyclin, histamine, pentagastrin and PL-10 substances (synthesized parts of BPC). At the end of this incubation cells were treated by 15% ethanol for 5 min. Cell viability was tested by trypan blue exclusion test and succinic dehydrogenase activity. The following results were obtained: I)prostacyclin, histamine and pentagastrin had no direct cytoprotective effect on isolated cells: and 2) PL-10 substances significantly protected the cells against ethanol-induced cellular damage. This led to the following conclusions: 1) in the phenomenon of gastric cytoprotection only the growth factor-like agents have a direct cellular effect; and 2) the intact peripheral innervation is basically necessary for the development of mediators and hormone-induced gastric cytoprotection.

Original languageEnglish
Pages (from-to)183-187
Number of pages5
JournalJournal of Physiology Paris
Volume91
Issue number3-5
DOIs
Publication statusPublished - Jan 1 1997

Keywords

  • Cryoprotective agents
  • Isolated gastric mucosal cells
  • Neural regulation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Direct cellular effects of some mediators, hormones and growth factor-like agents on denervated (isolated) rat gastric mucosal cells'. Together they form a unique fingerprint.

  • Cite this