Direct bioautography as a high-throughput screening method for the detection of antibacterial components from plant sources

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11 Citations (Scopus)

Abstract

The applicability of direct bioautography, the combination of planar chromatography with antimicrobial assay, is demonstrated with special emphasis on its recent developments such as BioArena and the use of genetically modified luminescent bacteria. Its methodological advancement is put into a historical perspective. In comparison with other commonly used antimicrobial susceptibility tests, the main advantage of direct bioautography resides in its simplicity, rapidity, and ability to detect separated individual matrix components exhibiting antimicrobial activity in situ. It is confirmed with examples that high-throughput direct bioautography is suitable as a biomonitoringscreening system for bioassay-guided isolation.

Original languageEnglish
Pages (from-to)850-856
Number of pages7
JournalJournal of AOAC International
Volume98
Issue number4
DOIs
Publication statusPublished - Jul 1 2015

Fingerprint

High-Throughput Screening Assays
Plant Structures
antimicrobial activity
Bioassay
historical perspective
Chromatography
Biological Assay
chromatography
bioassay
Assays
Bacteria
Screening
anti-infective agents
Throughput
assay
screening
bacterium
matrix
bioassays
methodology

ASJC Scopus subject areas

  • Analytical Chemistry
  • Agronomy and Crop Science
  • Food Science
  • Environmental Chemistry
  • Pharmacology
  • Medicine(all)

Cite this

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