Direct β-adrenergic stimulation of aldosterone secretion in cultured bovine adrenal subcapsular cells

A. De Lean, K. Rácz, N. McNicoll, M. L. Desrosiers

Research output: Contribution to journalArticle

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Abstract

Primary culture of bovine adrenal subcapsular cells was used to investigate direct effects of catecholamines on aldosterone secretion. Cells dispersed with collagenase and DNAse and cultured at high density (1.5-2 million/ml) for 3 days displayed high sensitivity to angiotensin II and ACTH, with an ED50 of 1.4 and 1.5 nM, respectively. Adrenergic agonists elicited a 4- to 6-fold stimulation of aldosterone secretion with potency order (-)isoproterenol greater than (-)epinephrine equals (-)norepinephrine greater than (+)isoproterenol, and corresponding ED50 5, 240, 213, and 3000 nM, respectively. No reproducible inhibition by dopamine of basal or stimulated levels of aldosterone secretion could be detected, but a weak stimulatory effect was sometimes observed at high concentration greater than 10 μM. (-)Isoproterenol stimulation of aldosterone production was potently inhibited by the β-adrenergic antagonists (-)alprenolol and (+)alprenolol with potencies of 1.8 and 110 nM, respectively. The α-adrenergic antagonists prazosin, yohimbine, and phentolamine only weakly inhibited (-)isoproterenol stimulation with potencies of 5, 13, and 140 μM, respectively. The potent β2-adrenergic antagonist ICI 118.551 and the weaker β1-adrenergic antagonist atenolol were roughly equipotent with potencies of 0.27 and 0.44 μM, respectively. Addition of the phosphodiesterase inhibitor Ro 20-1724 at 10 μM doubled the maximum stimulation effect of (-)isoproterenol without changing the potency of the catecholamine or the basal level of aldosterone secretion, suggesting a potential role of cAMP as a mediator of isoproterenol stimulation. These results indicate the presence of a β1-adrenergic receptor stimulating aldosterone secretion in bovine zona glomerulosa cells. The physiological significance of direct β-adrenergic stimulation of aldosterone production is currently being assessed.

Original languageEnglish
Pages (from-to)485-492
Number of pages8
JournalEndocrinology
Volume115
Issue number2
Publication statusPublished - 1984

Fingerprint

Aldosterone
Adrenergic Agents
Isoproterenol
Adrenergic Antagonists
Alprenolol
Catecholamines
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone
Zona Glomerulosa
Adrenergic Agonists
Atenolol
Phosphodiesterase Inhibitors
Yohimbine
Prazosin
Phentolamine
Collagenases
Angiotensin II
Adrenocorticotropic Hormone
Adrenergic Receptors
Epinephrine
Dopamine

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Direct β-adrenergic stimulation of aldosterone secretion in cultured bovine adrenal subcapsular cells. / De Lean, A.; Rácz, K.; McNicoll, N.; Desrosiers, M. L.

In: Endocrinology, Vol. 115, No. 2, 1984, p. 485-492.

Research output: Contribution to journalArticle

De Lean, A. ; Rácz, K. ; McNicoll, N. ; Desrosiers, M. L. / Direct β-adrenergic stimulation of aldosterone secretion in cultured bovine adrenal subcapsular cells. In: Endocrinology. 1984 ; Vol. 115, No. 2. pp. 485-492.
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