Diploid formation of Candida tropicalis via protoplast fusion

C. Vallin, L. Ferenczy

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Haploid auxotrophic mutants were produced from Candida tropicalis, and protoplast fusion was induced by polyethylene glycol. The resulting nutritional complementation was due to heterokaryon formation and, at a much lower frequency, to spontaneous diploidization. During cultivation, heterokaryotic clones regularly gave rise to heterozygous diploids from which in turn, haploids could be isolated. The technique of protoplast fusion gives an opportunity for genetic analysis of this and similar asexual fungal species.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalActa microbiologica Academiae Scientiarum Hungaricae
Volume25
Issue number3
Publication statusPublished - Dec 1 1978

ASJC Scopus subject areas

  • Medicine(all)

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