Dihydrolipoic acid reduces cytochrome b561 proteins

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cytochrome b561 (Cyt-b561) proteins constitute a family of trans-membrane proteins that are present in a wide variety of organisms. Two of their characteristic properties are the reducibility by ascorbate (ASC) and the presence of two distinct b-type hemes localized on two opposite sides of the membrane. Here we show that the tonoplast-localized and the putative tumor suppressor Cyt-b561 proteins can be reduced by other reductants than ASC and dithionite. A detailed spectral analysis of the ASC-dependent and dihydrolipoic acid (DHLA)-dependent reduction of these two Cyt-b561 proteins is also presented. Our results are discussed in relation to the known antioxidant capability of DHLA as well as its role in the regeneration of other antioxidant compounds of cells. These results allow us to speculate on new biological functions for the trans-membrane Cyt-b561 proteins.

Original languageEnglish
Pages (from-to)159-168
Number of pages10
JournalEuropean Biophysics Journal
Volume42
Issue number2-3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Proteins
Antioxidants
Dithionite
Membranes
Reducing Agents
Heme
Regeneration
Membrane Proteins
dihydrolipoic acid
cytochrome b561
Neoplasms

Keywords

  • Antioxidants
  • Ascorbate
  • Cytochrome b561 proteins
  • Dihydrolipoic acid
  • SVD analysis
  • UV-Vis

ASJC Scopus subject areas

  • Biophysics

Cite this

Dihydrolipoic acid reduces cytochrome b561 proteins. / Bérczi, A.; Zimányi, L.; Asard, Han.

In: European Biophysics Journal, Vol. 42, No. 2-3, 03.2013, p. 159-168.

Research output: Contribution to journalArticle

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