Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes

Sashka Boychova Krumova, Sergey Petrovich Laptenok, L. Kovács, Tünde Tóth, Arie van Hoek, G. Garab, Herbert van Amerongen

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type-WT) occur at 4-7°C lower temperatures in dgd1. Similar differences are revealed in the temperature dependence of the lipid packing and the membrane permeability: at elevated temperatures MC540 appears to be extruded from the dgd1 membrane bilayer around 35°C, whereas in WT, it remains lipid-bound up to 45°C and dgd1 and WT membranes become leaky around 35 and 45°C, respectively. It is concluded that DGDG plays important roles in the overall organization of thylakoid membranes especially at elevated temperatures.

Original languageEnglish
Pages (from-to)229-242
Number of pages14
JournalPhotosynthesis Research
Volume105
Issue number3
DOIs
Publication statusPublished - 2010

Fingerprint

Thylakoids
Diglycerides
diacylglycerols
thermal stability
thylakoids
Thermodynamic stability
Hot Temperature
Membranes
chlorophyll
Temperature
Fluorescence
fluorescence
Chlorophyll
Lipids
temperature
lipids
circular dichroism spectroscopy
Photosystem I Protein Complex
light harvesting complex
photosystem I

Keywords

  • Arabidopsis mutants
  • Dgd1 mutant
  • Digalactosyl-diacylglycerol
  • Thermal stability
  • Thylakoid lipids
  • Thylakoid membranes

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry

Cite this

Krumova, S. B., Laptenok, S. P., Kovács, L., Tóth, T., van Hoek, A., Garab, G., & van Amerongen, H. (2010). Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes. Photosynthesis Research, 105(3), 229-242. https://doi.org/10.1007/s11120-010-9581-5

Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes. / Krumova, Sashka Boychova; Laptenok, Sergey Petrovich; Kovács, L.; Tóth, Tünde; van Hoek, Arie; Garab, G.; van Amerongen, Herbert.

In: Photosynthesis Research, Vol. 105, No. 3, 2010, p. 229-242.

Research output: Contribution to journalArticle

Krumova, SB, Laptenok, SP, Kovács, L, Tóth, T, van Hoek, A, Garab, G & van Amerongen, H 2010, 'Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes', Photosynthesis Research, vol. 105, no. 3, pp. 229-242. https://doi.org/10.1007/s11120-010-9581-5
Krumova, Sashka Boychova ; Laptenok, Sergey Petrovich ; Kovács, L. ; Tóth, Tünde ; van Hoek, Arie ; Garab, G. ; van Amerongen, Herbert. / Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes. In: Photosynthesis Research. 2010 ; Vol. 105, No. 3. pp. 229-242.
@article{1e3f5f86747740219155eae07f382e5b,
title = "Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes",
abstract = "We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type-WT) occur at 4-7°C lower temperatures in dgd1. Similar differences are revealed in the temperature dependence of the lipid packing and the membrane permeability: at elevated temperatures MC540 appears to be extruded from the dgd1 membrane bilayer around 35°C, whereas in WT, it remains lipid-bound up to 45°C and dgd1 and WT membranes become leaky around 35 and 45°C, respectively. It is concluded that DGDG plays important roles in the overall organization of thylakoid membranes especially at elevated temperatures.",
keywords = "Arabidopsis mutants, Dgd1 mutant, Digalactosyl-diacylglycerol, Thermal stability, Thylakoid lipids, Thylakoid membranes",
author = "Krumova, {Sashka Boychova} and Laptenok, {Sergey Petrovich} and L. Kov{\'a}cs and T{\"u}nde T{\'o}th and {van Hoek}, Arie and G. Garab and {van Amerongen}, Herbert",
year = "2010",
doi = "10.1007/s11120-010-9581-5",
language = "English",
volume = "105",
pages = "229--242",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes

AU - Krumova, Sashka Boychova

AU - Laptenok, Sergey Petrovich

AU - Kovács, L.

AU - Tóth, Tünde

AU - van Hoek, Arie

AU - Garab, G.

AU - van Amerongen, Herbert

PY - 2010

Y1 - 2010

N2 - We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type-WT) occur at 4-7°C lower temperatures in dgd1. Similar differences are revealed in the temperature dependence of the lipid packing and the membrane permeability: at elevated temperatures MC540 appears to be extruded from the dgd1 membrane bilayer around 35°C, whereas in WT, it remains lipid-bound up to 45°C and dgd1 and WT membranes become leaky around 35 and 45°C, respectively. It is concluded that DGDG plays important roles in the overall organization of thylakoid membranes especially at elevated temperatures.

AB - We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type-WT) occur at 4-7°C lower temperatures in dgd1. Similar differences are revealed in the temperature dependence of the lipid packing and the membrane permeability: at elevated temperatures MC540 appears to be extruded from the dgd1 membrane bilayer around 35°C, whereas in WT, it remains lipid-bound up to 45°C and dgd1 and WT membranes become leaky around 35 and 45°C, respectively. It is concluded that DGDG plays important roles in the overall organization of thylakoid membranes especially at elevated temperatures.

KW - Arabidopsis mutants

KW - Dgd1 mutant

KW - Digalactosyl-diacylglycerol

KW - Thermal stability

KW - Thylakoid lipids

KW - Thylakoid membranes

UR - http://www.scopus.com/inward/record.url?scp=78149467077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149467077&partnerID=8YFLogxK

U2 - 10.1007/s11120-010-9581-5

DO - 10.1007/s11120-010-9581-5

M3 - Article

VL - 105

SP - 229

EP - 242

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 3

ER -