Differing effects of cardioplegic solution on bovine aortic v pulmonary arterial endothelial cell (EC) ACE activity

I. Horváth, C. Snead, J. D. Catravas

Research output: Contribution to journalArticle

Abstract

We have previously reported (Circulation 94:162, 1996) that EC angiotensin converting enzyme (ACE) activity increases following coronary arterial bypass grafting, in vivo. To investigate possible mechanisms of this effect, we studied the role of the high potassium (HP) and low potassium (LP) containing Plegisol cardioplegic solutions on EC ACE activity, in 24-well plates monolayers, in vitro, using two ontogenetically different EC lines: bovine aortic EC (BAEC) and bovine pulmonary arterial EC (BPAEC). EC were exposed to M199 cell culture medium (control), HP or LP (20% in M199) for 1, 2 or 4 h 28°C. After the cold cardioplegic incubation period, ACE activity was measured using the synthetic ACE substrate, 3H-benzoyl-Phe-Ala-Pro (0 3μCi incubated in 600μl Earle's balanced salt solution for 45 min at 37°C). In BPAEC, ACE activity significantly increased after 1 (1.39 v. 2 65 U/well), 2 (0.23 v. 0.35 U/well) and 4 h (0.45 v 0.56 U/well) exposure to LP, as well as after 1 (1.39 v. 1.83 U/well), 2 (0.23 v. 0.26 U/well), but not 4 h (0.45 v. 0.3 U/well) exposure to HP. In BAEC, small increases in ACE activity were observed only after 2 (0.04 v. 0.1 U/well) and 4 h exposure to LP (0.144 v 0.181 U/well) and 2h exposure to HP (0.04 v. 0.069 U/well). We conclude that ontogenetically different EC respond differently to cardioplegic solutions, in culture.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number3
Publication statusPublished - 1997

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Cardioplegic Solutions
Endothelial cells
peptidyl-dipeptidase A
Enzyme activity
Peptidyl-Dipeptidase A
endothelial cells
Potassium
Endothelial Cells
lungs
potassium
enzyme activity
Lung
cattle
Cell culture
enzyme substrates
Culture Media
Monolayers
cell culture
Cell Culture Techniques
Salts

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Differing effects of cardioplegic solution on bovine aortic v pulmonary arterial endothelial cell (EC) ACE activity. / Horváth, I.; Snead, C.; Catravas, J. D.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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