Differentiation-regulated expression of toll-like receptors 2 and 4 in HaCat keratinocytes

Andor Pivarcsi, A. Koreck, L. Bodai, M. Széll, Csilla Szeg, Nóra Belso, Anna Kenderessy-Szabó, Z. Bata-Csörgő, A. Dobozy, L. Kemény

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene (r=0.809, P

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalArchives of Dermatological Research
Volume296
Issue number3
DOIs
Publication statusPublished - Aug 2004

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Toll-Like Receptor 2
Toll-Like Receptor 4
Keratinocytes
Real-Time Polymerase Chain Reaction
Genes
Toll-Like Receptors
Flow Cytometry
Gene Expression
involucrin

Keywords

  • Epidermis
  • Host defense
  • Innate immunity
  • Keratinocyte
  • Toll-like receptor

ASJC Scopus subject areas

  • Dermatology

Cite this

Differentiation-regulated expression of toll-like receptors 2 and 4 in HaCat keratinocytes. / Pivarcsi, Andor; Koreck, A.; Bodai, L.; Széll, M.; Szeg, Csilla; Belso, Nóra; Kenderessy-Szabó, Anna; Bata-Csörgő, Z.; Dobozy, A.; Kemény, L.

In: Archives of Dermatological Research, Vol. 296, No. 3, 08.2004, p. 120-124.

Research output: Contribution to journalArticle

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AU - Koreck, A.

AU - Bodai, L.

AU - Széll, M.

AU - Szeg, Csilla

AU - Belso, Nóra

AU - Kenderessy-Szabó, Anna

AU - Bata-Csörgő, Z.

AU - Dobozy, A.

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AB - Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene (r=0.809, P

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