Differentiation of potato (Solanum tuberosum L.) plants from cultured leaf protoplasts.

A. Fehér, J. Preiszner, D. Dudits

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Through induction of totipotent stage in cultured cells plants were regenerated from protoplast derived callus colonies of potato. Growing of the plants in vitro with optimal aeration and pretreatment of the leaves in dark and cold prior to protoplast isolation has improved the protoplast yield and frequency of cell division. Protoplasts of four potato genotypes have started to divide within 2-5 days after plating them into culture medium containing 2,4-D (0.2 mg/l); ZEA (0.5 mg/l); and NAA (1 mg/l) as growth regulators. Embedding of the cells into agarose proved to be favourable to avoid cell browning and to increase colony formation. The series of hormone treatments based on complex action of NAA and BAP promoting colony growth and greening, ZEA and IAA inducing shoot redifferentiation, and GA3 plus NAA supporting shoot elongation and rooting, finally resulted in high frequency of plant regeneration from microcolonies.

Original languageEnglish
Pages (from-to)369-380
Number of pages12
JournalActa Biologica Hungarica
Volume40
Issue number4
Publication statusPublished - 1989

Fingerprint

Plant Leaves
Protoplasts
neutron activation analysis
Solanum tuberosum
potato
protoplasts
naphthaleneacetic acid
potatoes
2,4-Dichlorophenoxyacetic Acid
shoot
Plating
Sepharose
Culture Media
leaves
Elongation
growth regulator
2,4 dichlorophenoxyacetic acid
Cells
Hormones
rooting

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Differentiation of potato (Solanum tuberosum L.) plants from cultured leaf protoplasts. / Fehér, A.; Preiszner, J.; Dudits, D.

In: Acta Biologica Hungarica, Vol. 40, No. 4, 1989, p. 369-380.

Research output: Contribution to journalArticle

@article{291ed6d8bb334e63829edbabb2afa9ba,
title = "Differentiation of potato (Solanum tuberosum L.) plants from cultured leaf protoplasts.",
abstract = "Through induction of totipotent stage in cultured cells plants were regenerated from protoplast derived callus colonies of potato. Growing of the plants in vitro with optimal aeration and pretreatment of the leaves in dark and cold prior to protoplast isolation has improved the protoplast yield and frequency of cell division. Protoplasts of four potato genotypes have started to divide within 2-5 days after plating them into culture medium containing 2,4-D (0.2 mg/l); ZEA (0.5 mg/l); and NAA (1 mg/l) as growth regulators. Embedding of the cells into agarose proved to be favourable to avoid cell browning and to increase colony formation. The series of hormone treatments based on complex action of NAA and BAP promoting colony growth and greening, ZEA and IAA inducing shoot redifferentiation, and GA3 plus NAA supporting shoot elongation and rooting, finally resulted in high frequency of plant regeneration from microcolonies.",
author = "A. Feh{\'e}r and J. Preiszner and D. Dudits",
year = "1989",
language = "English",
volume = "40",
pages = "369--380",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "4",

}

TY - JOUR

T1 - Differentiation of potato (Solanum tuberosum L.) plants from cultured leaf protoplasts.

AU - Fehér, A.

AU - Preiszner, J.

AU - Dudits, D.

PY - 1989

Y1 - 1989

N2 - Through induction of totipotent stage in cultured cells plants were regenerated from protoplast derived callus colonies of potato. Growing of the plants in vitro with optimal aeration and pretreatment of the leaves in dark and cold prior to protoplast isolation has improved the protoplast yield and frequency of cell division. Protoplasts of four potato genotypes have started to divide within 2-5 days after plating them into culture medium containing 2,4-D (0.2 mg/l); ZEA (0.5 mg/l); and NAA (1 mg/l) as growth regulators. Embedding of the cells into agarose proved to be favourable to avoid cell browning and to increase colony formation. The series of hormone treatments based on complex action of NAA and BAP promoting colony growth and greening, ZEA and IAA inducing shoot redifferentiation, and GA3 plus NAA supporting shoot elongation and rooting, finally resulted in high frequency of plant regeneration from microcolonies.

AB - Through induction of totipotent stage in cultured cells plants were regenerated from protoplast derived callus colonies of potato. Growing of the plants in vitro with optimal aeration and pretreatment of the leaves in dark and cold prior to protoplast isolation has improved the protoplast yield and frequency of cell division. Protoplasts of four potato genotypes have started to divide within 2-5 days after plating them into culture medium containing 2,4-D (0.2 mg/l); ZEA (0.5 mg/l); and NAA (1 mg/l) as growth regulators. Embedding of the cells into agarose proved to be favourable to avoid cell browning and to increase colony formation. The series of hormone treatments based on complex action of NAA and BAP promoting colony growth and greening, ZEA and IAA inducing shoot redifferentiation, and GA3 plus NAA supporting shoot elongation and rooting, finally resulted in high frequency of plant regeneration from microcolonies.

UR - http://www.scopus.com/inward/record.url?scp=0024933222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024933222&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 369

EP - 380

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 4

ER -