Differentiation of Mycoplasma gallisepticum strains using molecular methods

Judit Bíró, Noémi Erdel, Ibolya Székely, L. Stipkovits

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Increasing use of Mycoplasma gallisepticum (MG) live vaccines has led to a need for the differentiation of MG strains. The MG strains MK-7, MS-16, S6, FS-9 and R strains and the MG live vaccine strain F were compared by random amplification of polymorphic DNA (RAPD) in this study. Using RAPD, different patterns were found among the MG strains. In addition to this, we examined the differentiating potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) primers targeted at the crmA, crmB, crmC, gapA, mgc2 and pvpA genes encoding cytadherence-related surface proteins. These proteins may take part in the pathogenesis of MG-induced disease. Differentiation of strain F is based on the identification of restriction enzyme sites in the PCR amplicons. Using HphI enzyme, crmC PCR amplicons produced different RFLP patterns. Digestion of amplicons of gapA-specific PCR with MboI enzyme also produced distinct patterns. Differences were observed among strains R and F by digestion of mgc2 PCR amplicons with HaeIII and VspI enzymes and digestion of pvpA PCR amplicons with AccI, PvuII and ScrFI endonucleases. This method can be used for the rapid differentiation of vaccine strain from wild strains. Differentiation of MG strains is a great advantage for diagnosticians or practitioners and it is useful for epidemiological studies.

Original languageEnglish
Pages (from-to)437-438
Number of pages2
JournalActa Veterinaria Hungarica
Volume54
Issue number4
DOIs
Publication statusPublished - 2006

Fingerprint

Mycoplasma gallisepticum
Polymerase Chain Reaction
Digestion
Vaccines
Enzymes
Restriction Fragment Length Polymorphisms
methodology
digestion
live vaccines
enzymes
S 6
restriction fragment length polymorphism
DNA
Epidemiologic Studies
Membrane Proteins
surface proteins
epidemiological studies
pathogenesis
polymerase chain reaction

Keywords

  • Mycoplasma gallisepticum
  • PCR-RFLP
  • RAPD PCR
  • Vaccine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Differentiation of Mycoplasma gallisepticum strains using molecular methods. / Bíró, Judit; Erdel, Noémi; Székely, Ibolya; Stipkovits, L.

In: Acta Veterinaria Hungarica, Vol. 54, No. 4, 2006, p. 437-438.

Research output: Contribution to journalArticle

Bíró, Judit ; Erdel, Noémi ; Székely, Ibolya ; Stipkovits, L. / Differentiation of Mycoplasma gallisepticum strains using molecular methods. In: Acta Veterinaria Hungarica. 2006 ; Vol. 54, No. 4. pp. 437-438.
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