Differentiation of grapevine (Vitis vinifera L.) conculta members based on molecular tools

Peter Bodor, Antal Szoke, Kitti Toth-Lencses, Aniko Veres, Tamas Deak, Pal Kozma, Gyorgy Denes Bisztray, Erzsebet Kiss

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Twenty-seven grapevine (Vitis vinifera L.) varieties within 12 putative berry colour variation groups (conculta) were genotyped with 14 highly polymorphic microsatellite (simple sequence repeats (SSR)) markers. Three additional oligonucleotide primers were applied for the detection of the Gret1 retroelement insertion in the promoter region of VvMybA1 transcription factor gene regulating the UFGT (UDP-glucose: flavonoid 3-O-glucosyltransferase) activity. UFGT is the key enzyme of the anthocyanin biosynthetic pathway. SSR results proved that the analysed cultivars can be grouped only into nine concultas, the other three putative berry colour variant groups consist of homonyms as a consequence of misnaming. In the case of Sárfehér-Sárpiros, Delaware red-Delaware white and Járdovány fekete-Járdovány fehér, it was attested that they are not bud sports, but homonyms. Some conculta members could be differentiated according to the presence or the absence of the Gret1 retroelement (Chasselas, Furmint and Lisztes), while others, Bajor, Bakator, Gohér and Traminer conculta members, remained indistinguishable either by the microsatellites or the Gret1-based method.

Original languageEnglish
Pages (from-to)14-20
Number of pages7
JournalBiotechnology and Biotechnological Equipment
Volume28
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Conculta
  • Gret1 retroelement
  • SSR
  • VvMybA1

ASJC Scopus subject areas

  • Biotechnology

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