Differentiation of adipocytes in monolayer from mouse embryonic stem cells

Ixchelt Cuaranta-Monroy, Zoltan Simandi, Laszlo Nagy

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Obesity and its comorbidity incidence have increased worldwide during the past 10 years. In consequence, researchers have drawn their attention to the understanding of adipocyte differentiation. Several cellular model systems have been established; however no efficient protocol could be developed so far to differentiate the pluripotent embryonic stem cells to adipocytes. In this chapter, we describe a detailed protocol that is optimized for mouse embryonic stem cells. The result of this differentiation is a homogenous adipocyte monolayer culture that can be used for several applications including developmental and pharmacological research.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages407-415
Number of pages9
DOIs
Publication statusPublished - Oct 1 2016

Publication series

NameMethods in Molecular Biology
Volume1341
ISSN (Print)1064-3745

Keywords

  • Adipocyte differentiation
  • Ascorbic acid
  • Monolayer culture
  • Mouse embryonic stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Cuaranta-Monroy, I., Simandi, Z., & Nagy, L. (2016). Differentiation of adipocytes in monolayer from mouse embryonic stem cells. In Methods in Molecular Biology (pp. 407-415). (Methods in Molecular Biology; Vol. 1341). Humana Press Inc.. https://doi.org/10.1007/7651_2015_219