Differentially expressed genes associated with human limbal epithelial phenotypes

New molecules that potentially facilitate selection of stem cell-enriched populations

Lili Takács, Eniko Tóth, Gergely Losonczy, Attila Szanto, Tomi Bähr-Ivacevic, Vladimir Benes, A. Berta, G. Vereb

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

PURPOSE. The aim of this study was to identify differentially expressed genes in the human limbal epithelium by microarray analysis. METHODS. Total RNA isolates of human limbal and central corneal epithelia were used after transcription for hybridization on whole human genome expression microarrays. A set of differentially expressed genes detected by both microarrays was established. In the case of eight selected molecules, microarray results were confirmed by qRT-PCR, and protein expression in the cornea was examined by confocal immunofluorescence microscopy. Colocalization with the putative stem cell marker C/EBPδ was also examined. RESULTS. The authors established a database of 126 limbal overexpressed genes. qRT-PCR confirmed microarray results in all examined cases (SPON1, IFITM1, ITM2A, PHLDA1, CXCR4, FZD7, DCT, DKK4). Limbal localization of the protein product of SPON1, IFITM1, ITM2A, CXCR4, and DKK4 was shown with confocal immunofluorescence microscopy. SPON1, IFITM1, and ITM2A signals mostly colocalized with C/EBPγ-positive putative resting limbal stem cells. CONCLUSIONS. By detecting several new differentially expressed genes in the human corneal limbus, this study further expands current knowledge on the molecular signature of limbal epithelial stem cells. Plasma membrane localization of IFITM1 and ITM2A suggests their potential usefulness as targets to select stem cell-enriched populations from the limbal epithelium.

Original languageEnglish
Pages (from-to)1252-1260
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number3
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Stem Cells
Phenotype
Fluorescence Microscopy
Confocal Microscopy
Population
Genes
Epithelium
Limbus Corneae
Polymerase Chain Reaction
Corneal Epithelium
Human Genome
Microarray Analysis
Cornea
Proteins
Epithelial Cells
Cell Membrane
Databases
RNA

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Differentially expressed genes associated with human limbal epithelial phenotypes : New molecules that potentially facilitate selection of stem cell-enriched populations. / Takács, Lili; Tóth, Eniko; Losonczy, Gergely; Szanto, Attila; Bähr-Ivacevic, Tomi; Benes, Vladimir; Berta, A.; Vereb, G.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 3, 03.2011, p. 1252-1260.

Research output: Contribution to journalArticle

@article{1379270ceadc405497c84f3446d086e4,
title = "Differentially expressed genes associated with human limbal epithelial phenotypes: New molecules that potentially facilitate selection of stem cell-enriched populations",
abstract = "PURPOSE. The aim of this study was to identify differentially expressed genes in the human limbal epithelium by microarray analysis. METHODS. Total RNA isolates of human limbal and central corneal epithelia were used after transcription for hybridization on whole human genome expression microarrays. A set of differentially expressed genes detected by both microarrays was established. In the case of eight selected molecules, microarray results were confirmed by qRT-PCR, and protein expression in the cornea was examined by confocal immunofluorescence microscopy. Colocalization with the putative stem cell marker C/EBPδ was also examined. RESULTS. The authors established a database of 126 limbal overexpressed genes. qRT-PCR confirmed microarray results in all examined cases (SPON1, IFITM1, ITM2A, PHLDA1, CXCR4, FZD7, DCT, DKK4). Limbal localization of the protein product of SPON1, IFITM1, ITM2A, CXCR4, and DKK4 was shown with confocal immunofluorescence microscopy. SPON1, IFITM1, and ITM2A signals mostly colocalized with C/EBPγ-positive putative resting limbal stem cells. CONCLUSIONS. By detecting several new differentially expressed genes in the human corneal limbus, this study further expands current knowledge on the molecular signature of limbal epithelial stem cells. Plasma membrane localization of IFITM1 and ITM2A suggests their potential usefulness as targets to select stem cell-enriched populations from the limbal epithelium.",
author = "Lili Tak{\'a}cs and Eniko T{\'o}th and Gergely Losonczy and Attila Szanto and Tomi B{\"a}hr-Ivacevic and Vladimir Benes and A. Berta and G. Vereb",
year = "2011",
month = "3",
doi = "10.1167/iovs.10-5242",
language = "English",
volume = "52",
pages = "1252--1260",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - Differentially expressed genes associated with human limbal epithelial phenotypes

T2 - New molecules that potentially facilitate selection of stem cell-enriched populations

AU - Takács, Lili

AU - Tóth, Eniko

AU - Losonczy, Gergely

AU - Szanto, Attila

AU - Bähr-Ivacevic, Tomi

AU - Benes, Vladimir

AU - Berta, A.

AU - Vereb, G.

PY - 2011/3

Y1 - 2011/3

N2 - PURPOSE. The aim of this study was to identify differentially expressed genes in the human limbal epithelium by microarray analysis. METHODS. Total RNA isolates of human limbal and central corneal epithelia were used after transcription for hybridization on whole human genome expression microarrays. A set of differentially expressed genes detected by both microarrays was established. In the case of eight selected molecules, microarray results were confirmed by qRT-PCR, and protein expression in the cornea was examined by confocal immunofluorescence microscopy. Colocalization with the putative stem cell marker C/EBPδ was also examined. RESULTS. The authors established a database of 126 limbal overexpressed genes. qRT-PCR confirmed microarray results in all examined cases (SPON1, IFITM1, ITM2A, PHLDA1, CXCR4, FZD7, DCT, DKK4). Limbal localization of the protein product of SPON1, IFITM1, ITM2A, CXCR4, and DKK4 was shown with confocal immunofluorescence microscopy. SPON1, IFITM1, and ITM2A signals mostly colocalized with C/EBPγ-positive putative resting limbal stem cells. CONCLUSIONS. By detecting several new differentially expressed genes in the human corneal limbus, this study further expands current knowledge on the molecular signature of limbal epithelial stem cells. Plasma membrane localization of IFITM1 and ITM2A suggests their potential usefulness as targets to select stem cell-enriched populations from the limbal epithelium.

AB - PURPOSE. The aim of this study was to identify differentially expressed genes in the human limbal epithelium by microarray analysis. METHODS. Total RNA isolates of human limbal and central corneal epithelia were used after transcription for hybridization on whole human genome expression microarrays. A set of differentially expressed genes detected by both microarrays was established. In the case of eight selected molecules, microarray results were confirmed by qRT-PCR, and protein expression in the cornea was examined by confocal immunofluorescence microscopy. Colocalization with the putative stem cell marker C/EBPδ was also examined. RESULTS. The authors established a database of 126 limbal overexpressed genes. qRT-PCR confirmed microarray results in all examined cases (SPON1, IFITM1, ITM2A, PHLDA1, CXCR4, FZD7, DCT, DKK4). Limbal localization of the protein product of SPON1, IFITM1, ITM2A, CXCR4, and DKK4 was shown with confocal immunofluorescence microscopy. SPON1, IFITM1, and ITM2A signals mostly colocalized with C/EBPγ-positive putative resting limbal stem cells. CONCLUSIONS. By detecting several new differentially expressed genes in the human corneal limbus, this study further expands current knowledge on the molecular signature of limbal epithelial stem cells. Plasma membrane localization of IFITM1 and ITM2A suggests their potential usefulness as targets to select stem cell-enriched populations from the limbal epithelium.

UR - http://www.scopus.com/inward/record.url?scp=79955943286&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955943286&partnerID=8YFLogxK

U2 - 10.1167/iovs.10-5242

DO - 10.1167/iovs.10-5242

M3 - Article

VL - 52

SP - 1252

EP - 1260

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -