Differential scanning calorimetric examination of the ruptured Achilles tendon in human

N. Wiegand, L. Vámhidy, L. Kereskai, D. Lőrinczy

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Achilles tendon rupture is a common injury of the foot in middle age and physically active population in Europe. The aetiology of the degenerative changes in the collagen structures of the tendon which could be disposed for the rupture is still not clear. Our hypothesis was that before the injury there is a clear pathological abnormality in the tissue elements building up the Achilles tendon, which is responsible for the disease, and could be monitored besides the classical histological methods by differential scanning calorimetry. The thermal denaturation of human samples was monitored by a SETARAM Micro DSC-II calorimeter. All the experiments were performed between 0 and 100 °C. The heating rate was 0.3 K/min. DSC scans clearly demonstrated significant differences between the control and ruptured samples (control: Tm = 59.7 °C, T1/2 = 1.4 °C and ΔHcal = 8.54 J/g; ruptured: Tm = 62.75 °C, T1/2 = 2.6 °C and ΔHcal = 1.54 J/g). These observations could be explained with the structural alterations caused by the biochemical and structural processes. With our investigations we could demonstrate that DSC is a useful and well applicable method for the investigation of collagen tissue of the human Achilles tendon. We can prove with this method, that the earlier series of microtraumas which result a scar formation in the tendon tissue increases the thermal stability of collagen in ruptured tendon.

Original languageEnglish
Pages (from-to)7-10
Number of pages4
JournalThermochimica Acta
Volume498
Issue number1-2
DOIs
Publication statusPublished - Jan 20 2010

Fingerprint

tendons
Tendons
examination
Scanning
scanning
collagens
Collagen
Tissue
etiology
scars
Denaturation
biopolymer denaturation
abnormalities
Calorimeters
Heating rate
calorimeters
Differential scanning calorimetry
Thermodynamic stability
thermal stability
heat measurement

Keywords

  • Achilles tendon rupture
  • DSC

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics
  • Instrumentation

Cite this

Differential scanning calorimetric examination of the ruptured Achilles tendon in human. / Wiegand, N.; Vámhidy, L.; Kereskai, L.; Lőrinczy, D.

In: Thermochimica Acta, Vol. 498, No. 1-2, 20.01.2010, p. 7-10.

Research output: Contribution to journalArticle

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