Differential responses in vasopressin and oxytocin gene expression in distinct hypothalamic nuclei after hypothalamoneurohypophyseal disconnection and vasopressin substitution

Hubert H M Van Tol, J. Kiss, J. Peter H Burbach

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A possible feedback of circulating vasopressin (VP) on the expression of its gene in the hypothalamoneurohypophyseal system was studied in ruts with depleted stores of neurohypophyseal hormones. The neural lobe of the pituitary gland was disconnected by anterolateral deafferentation of the basal hypothalamus using a Halasz knife cut. One group of lesioned animals was substituted continuously with VP using mini-osmopumps. After 1 week, the lesion had caused a complete disappearance of neurohypophyseal hormones from the neurointcrmediate pituitary and induced a 2-fold increase in water intake and an increase in plasma osmolality of approximately 7 mOsm/kg. VP mRNA levels of the supraoptic nucleus (SON), paraventricular nucleus (PVN) and suprachiasmatic nucleus (SCN) were measured in punched tissue by Northern blot analysis and dot-blot analysis. VP mRNA levels of the SON and PVN were reduced by 50% due to the deafferentation. which might have been caused by degeneration. VP substitution of the lesioned animals normalised water intake but not plasma osmolality. In the VP-substituted animals, VP mRNA levels of the SON were 2-fold reduced compared to the nonsubstituted lesioned animals. The VP mRNA levels of the PVN were not affected by VP substitution. The VP mRNA levels of the SCN increased 2-fold after the lesion but were not influenced by VP substitution. Concomitant determination of oxytocin (OT) mRNA showed that OT mRNA levels in the PVN and SON were not affected by the deafferentation and did not respond to VP substitution. The results indicate that VP mRNA levels of the SON in hypotha-lamoneurohypophyseal-disconnected rats are negatively influenced by peripheral VP independently from changes in plasma osmolality. The data demonstrate that VP neurons of different hypothalamic nuclei display differential responses in VP gene expression. Furthermore, the results point to a different mechanism of regulation for VP and OT gene expression upon VP substitution and deafferentation.

Original languageEnglish
Pages (from-to)337-343
Number of pages7
JournalNeuroendocrinology
Volume49
Issue number4
DOIs
Publication statusPublished - 1989

Fingerprint

Oxytocin
Vasopressins
Gene Expression
Supraoptic Nucleus
Messenger RNA
Paraventricular Hypothalamic Nucleus
Osmolar Concentration
Posterior Pituitary Hormones
Suprachiasmatic Nucleus
Drinking
Posterior Pituitary Gland
Pituitary Gland

Keywords

  • Gene expression
  • Hypothalamoneurohypophyseal disconnection
  • Oxytocin
  • Paraventricular nucleus
  • Suprachiusmatic nucleus
  • Supraoptic nucleus
  • Vasopressin
  • Vasopressin substitution

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Differential responses in vasopressin and oxytocin gene expression in distinct hypothalamic nuclei after hypothalamoneurohypophyseal disconnection and vasopressin substitution. / Van Tol, Hubert H M; Kiss, J.; Burbach, J. Peter H.

In: Neuroendocrinology, Vol. 49, No. 4, 1989, p. 337-343.

Research output: Contribution to journalArticle

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abstract = "A possible feedback of circulating vasopressin (VP) on the expression of its gene in the hypothalamoneurohypophyseal system was studied in ruts with depleted stores of neurohypophyseal hormones. The neural lobe of the pituitary gland was disconnected by anterolateral deafferentation of the basal hypothalamus using a Halasz knife cut. One group of lesioned animals was substituted continuously with VP using mini-osmopumps. After 1 week, the lesion had caused a complete disappearance of neurohypophyseal hormones from the neurointcrmediate pituitary and induced a 2-fold increase in water intake and an increase in plasma osmolality of approximately 7 mOsm/kg. VP mRNA levels of the supraoptic nucleus (SON), paraventricular nucleus (PVN) and suprachiasmatic nucleus (SCN) were measured in punched tissue by Northern blot analysis and dot-blot analysis. VP mRNA levels of the SON and PVN were reduced by 50{\%} due to the deafferentation. which might have been caused by degeneration. VP substitution of the lesioned animals normalised water intake but not plasma osmolality. In the VP-substituted animals, VP mRNA levels of the SON were 2-fold reduced compared to the nonsubstituted lesioned animals. The VP mRNA levels of the PVN were not affected by VP substitution. The VP mRNA levels of the SCN increased 2-fold after the lesion but were not influenced by VP substitution. Concomitant determination of oxytocin (OT) mRNA showed that OT mRNA levels in the PVN and SON were not affected by the deafferentation and did not respond to VP substitution. The results indicate that VP mRNA levels of the SON in hypotha-lamoneurohypophyseal-disconnected rats are negatively influenced by peripheral VP independently from changes in plasma osmolality. The data demonstrate that VP neurons of different hypothalamic nuclei display differential responses in VP gene expression. Furthermore, the results point to a different mechanism of regulation for VP and OT gene expression upon VP substitution and deafferentation.",
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