Differential regulation of vasopressin gene expression in the hypothalamus of endotoxin-treated 14-day-old rat

András Boros, Péter Temesvári, László Szöke, Miklós Vecsernyés, Ferenc Bari, Csongor S. Ábrahám, Sándor Pintér, Karoly Gulya

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Abstract

The E. coli endotoxin 0111 B4, a lipopolysaccharide (LPS), in a dose of 200 ng/kg body weight/50 μl artificial cerebrospinal fluid (CSF) was given intracisternally to 14-day-old rats. Four hours later CSF, blood and urine were sampled, and consecutive brain sections from the hypothalamic area of the brain were prepared for in situ hybridization. The LPS treatment resulted in a significant (p < 0.001) pleocytosis and an elevation of the protein content of the CSF. There were no changes observed in the chemical parameters of the CSF, plasma, blood or urine, i.e. vasopressin (VP) levels, osmolality, Na+ and K+ concentrations, glucose level, pH, bicarbonate or PaCO2, PaO2 values. LPS injection, however, resulted in a significantly (p < 0.01) increased VP mRNA level (121% of the control value) in the supraoptic nuclei (SON), but not in the paraventricular nuclei (PVN), as compared to controls. Our findings suggest an early effect of LPS on VP gene expression selectively in the SON of 14-days-old rats. This animal model might be suitable for studying the regulation of VP gene expression and the role of this peptide in the pathogenesis of bacterial meningitis in pediatric patients.

Original languageEnglish
Pages (from-to)PL47-PL52
JournalLife Sciences
Volume65
Issue number4
DOIs
Publication statusPublished - Jun 18 1999

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Keywords

  • Bacterial endotoxin
  • Gene expression
  • Lipopolysaccharide
  • Rat
  • Supraoptic nuclei
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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