Differential regulation of vasopressin gene expression in the hypothalamus of endotoxin-treated 14-day-old rat

András Boros, Péter Temesvári, László Szöke, M. Vecsernyés, F. Bari, C. Ábrahám, S. Pintér, K. Gulya

Research output: Contribution to journalArticle

Abstract

The E. coli endotoxin 0111 B4, a lipopolysaccharide (LPS), in a dose of 200 ng/kg body weight/50 μl artificial cerebrospinal fluid (CSF) was given intracisternally to 14-day-old rats. Four hours later CSF, blood and urine were sampled, and consecutive brain sections from the hypothalamic area of the brain were prepared for in situ hybridization. The LPS treatment resulted in a significant (p <0.001) pleocytosis and an elevation of the protein content of the CSF. There were no changes observed in the chemical parameters of the CSF, plasma, blood or urine, i.e. vasopressin (VP) levels, osmolality, Na+ and K+ concentrations, glucose level, pH, bicarbonate or PaCO2, PaO2 values. LPS injection, however, resulted in a significantly (p <0.01) increased VP mRNA level (121% of the control value) in the supraoptic nuclei (SON), but not in the paraventricular nuclei (PVN), as compared to controls. Our findings suggest an early effect of LPS on VP gene expression selectively in the SON of 14-days-old rats. This animal model might be suitable for studying the regulation of VP gene expression and the role of this peptide in the pathogenesis of bacterial meningitis in pediatric patients.

Original languageEnglish
JournalLife Sciences
Volume65
Issue number4
DOIs
Publication statusPublished - Jun 18 1999

Fingerprint

Gene Expression Regulation
Cerebrospinal fluid
Vasopressins
Gene expression
Endotoxins
Hypothalamus
Lipopolysaccharides
Rats
Cerebrospinal Fluid
Supraoptic Nucleus
Brain
Blood
Urine
Cerebrospinal Fluid Proteins
Pediatrics
Bacterial Meningitides
Paraventricular Hypothalamic Nucleus
Leukocytosis
Bicarbonates
Osmolar Concentration

Keywords

  • Bacterial endotoxin
  • Gene expression
  • Lipopolysaccharide
  • Rat
  • Supraoptic nuclei
  • Vasopressin

ASJC Scopus subject areas

  • Pharmacology

Cite this

Differential regulation of vasopressin gene expression in the hypothalamus of endotoxin-treated 14-day-old rat. / Boros, András; Temesvári, Péter; Szöke, László; Vecsernyés, M.; Bari, F.; Ábrahám, C.; Pintér, S.; Gulya, K.

In: Life Sciences, Vol. 65, No. 4, 18.06.1999.

Research output: Contribution to journalArticle

@article{bfbd2647c92746f484637435551a8362,
title = "Differential regulation of vasopressin gene expression in the hypothalamus of endotoxin-treated 14-day-old rat",
abstract = "The E. coli endotoxin 0111 B4, a lipopolysaccharide (LPS), in a dose of 200 ng/kg body weight/50 μl artificial cerebrospinal fluid (CSF) was given intracisternally to 14-day-old rats. Four hours later CSF, blood and urine were sampled, and consecutive brain sections from the hypothalamic area of the brain were prepared for in situ hybridization. The LPS treatment resulted in a significant (p <0.001) pleocytosis and an elevation of the protein content of the CSF. There were no changes observed in the chemical parameters of the CSF, plasma, blood or urine, i.e. vasopressin (VP) levels, osmolality, Na+ and K+ concentrations, glucose level, pH, bicarbonate or PaCO2, PaO2 values. LPS injection, however, resulted in a significantly (p <0.01) increased VP mRNA level (121{\%} of the control value) in the supraoptic nuclei (SON), but not in the paraventricular nuclei (PVN), as compared to controls. Our findings suggest an early effect of LPS on VP gene expression selectively in the SON of 14-days-old rats. This animal model might be suitable for studying the regulation of VP gene expression and the role of this peptide in the pathogenesis of bacterial meningitis in pediatric patients.",
keywords = "Bacterial endotoxin, Gene expression, Lipopolysaccharide, Rat, Supraoptic nuclei, Vasopressin",
author = "Andr{\'a}s Boros and P{\'e}ter Temesv{\'a}ri and L{\'a}szl{\'o} Sz{\"o}ke and M. Vecserny{\'e}s and F. Bari and C. {\'A}brah{\'a}m and S. Pint{\'e}r and K. Gulya",
year = "1999",
month = "6",
day = "18",
doi = "10.1016/S0024-3205(99)00267-2",
language = "English",
volume = "65",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Differential regulation of vasopressin gene expression in the hypothalamus of endotoxin-treated 14-day-old rat

AU - Boros, András

AU - Temesvári, Péter

AU - Szöke, László

AU - Vecsernyés, M.

AU - Bari, F.

AU - Ábrahám, C.

AU - Pintér, S.

AU - Gulya, K.

PY - 1999/6/18

Y1 - 1999/6/18

N2 - The E. coli endotoxin 0111 B4, a lipopolysaccharide (LPS), in a dose of 200 ng/kg body weight/50 μl artificial cerebrospinal fluid (CSF) was given intracisternally to 14-day-old rats. Four hours later CSF, blood and urine were sampled, and consecutive brain sections from the hypothalamic area of the brain were prepared for in situ hybridization. The LPS treatment resulted in a significant (p <0.001) pleocytosis and an elevation of the protein content of the CSF. There were no changes observed in the chemical parameters of the CSF, plasma, blood or urine, i.e. vasopressin (VP) levels, osmolality, Na+ and K+ concentrations, glucose level, pH, bicarbonate or PaCO2, PaO2 values. LPS injection, however, resulted in a significantly (p <0.01) increased VP mRNA level (121% of the control value) in the supraoptic nuclei (SON), but not in the paraventricular nuclei (PVN), as compared to controls. Our findings suggest an early effect of LPS on VP gene expression selectively in the SON of 14-days-old rats. This animal model might be suitable for studying the regulation of VP gene expression and the role of this peptide in the pathogenesis of bacterial meningitis in pediatric patients.

AB - The E. coli endotoxin 0111 B4, a lipopolysaccharide (LPS), in a dose of 200 ng/kg body weight/50 μl artificial cerebrospinal fluid (CSF) was given intracisternally to 14-day-old rats. Four hours later CSF, blood and urine were sampled, and consecutive brain sections from the hypothalamic area of the brain were prepared for in situ hybridization. The LPS treatment resulted in a significant (p <0.001) pleocytosis and an elevation of the protein content of the CSF. There were no changes observed in the chemical parameters of the CSF, plasma, blood or urine, i.e. vasopressin (VP) levels, osmolality, Na+ and K+ concentrations, glucose level, pH, bicarbonate or PaCO2, PaO2 values. LPS injection, however, resulted in a significantly (p <0.01) increased VP mRNA level (121% of the control value) in the supraoptic nuclei (SON), but not in the paraventricular nuclei (PVN), as compared to controls. Our findings suggest an early effect of LPS on VP gene expression selectively in the SON of 14-days-old rats. This animal model might be suitable for studying the regulation of VP gene expression and the role of this peptide in the pathogenesis of bacterial meningitis in pediatric patients.

KW - Bacterial endotoxin

KW - Gene expression

KW - Lipopolysaccharide

KW - Rat

KW - Supraoptic nuclei

KW - Vasopressin

UR - http://www.scopus.com/inward/record.url?scp=0033580745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033580745&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(99)00267-2

DO - 10.1016/S0024-3205(99)00267-2

M3 - Article

VL - 65

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 4

ER -