Differential regulation of umbilical cord blood and leukemic B cells by interferon-alpha (IFN-α): Observations in cultured cells

István Szegedi, C. Kiss, Éva Karászi, G. Vámosi, J. Szöllősi, Péter Kovács, Ilona Benko

Research output: Contribution to journalArticle

Abstract

The exact mechanism of the beneficial therapeutic action of interferon-a (IFN-α) in B-cell-lineage malignancies has not been adequately explained. Here we report on the differential effect of IFN-α2b on non-malignant 6 cells of umbilical cord blood and leukemic B-cell lines JY, BL-41 and BCBL-1. Leukemic cell proliferation was characterized by colony assay, whereas apoptosis was investigated by flow cytometry of propidium iodide-stained cells. The degree of differentiation was evaluated by measuring the expression level of Fcγ receptor-II (FcγRII) labeled with anti-CD32-FITC monoclonal antibody using flow cytometry. IFN-α protected umbilical cord blood CD19-positive B lymphocytes from apoptotic cell death in vitro. IFN-α significantly decreased colony formation of all three cell lines, and in contrast to normal cells, induced apoptosis in JY and BL-41 and excessive necrosis in HHV-8 infected BCBL-1 cells. FcγRII was upregulated both in normal and in leukemic B cells as indicated by an increase both in the proportion of CD32-positive cells and the mean fluorescence intensity. From our results it seems that antiproliferative, apoptotic and differentiative effects of IFN-α are interrelated but distinct cellular events, which are differentially regulated in normal, leukemic and virus-infected cells of the B-cell lineage.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalPathology and Oncology Research
Volume12
Issue number3
Publication statusPublished - 2006

Fingerprint

Fetal Blood
Interferon-alpha
Cultured Cells
B-Lymphocytes
interferon alfa-2b
Fc Receptors
Cell Lineage
Flow Cytometry
Apoptosis
Cell Line
Human Herpesvirus 8
Propidium
Fluorescein-5-isothiocyanate
Interferons
Cell Death
Necrosis
Fluorescence
Monoclonal Antibodies
Cell Proliferation
Viruses

Keywords

  • Apoptosis
  • B cells
  • Colony formation
  • Interferon-alpha
  • Type II Fcγ receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Differential regulation of umbilical cord blood and leukemic B cells by interferon-alpha (IFN-α) : Observations in cultured cells. / Szegedi, István; Kiss, C.; Karászi, Éva; Vámosi, G.; Szöllősi, J.; Kovács, Péter; Benko, Ilona.

In: Pathology and Oncology Research, Vol. 12, No. 3, 2006, p. 159-163.

Research output: Contribution to journalArticle

@article{bdf0206db3884d449111044f0e1771fb,
title = "Differential regulation of umbilical cord blood and leukemic B cells by interferon-alpha (IFN-α): Observations in cultured cells",
abstract = "The exact mechanism of the beneficial therapeutic action of interferon-a (IFN-α) in B-cell-lineage malignancies has not been adequately explained. Here we report on the differential effect of IFN-α2b on non-malignant 6 cells of umbilical cord blood and leukemic B-cell lines JY, BL-41 and BCBL-1. Leukemic cell proliferation was characterized by colony assay, whereas apoptosis was investigated by flow cytometry of propidium iodide-stained cells. The degree of differentiation was evaluated by measuring the expression level of Fcγ receptor-II (FcγRII) labeled with anti-CD32-FITC monoclonal antibody using flow cytometry. IFN-α protected umbilical cord blood CD19-positive B lymphocytes from apoptotic cell death in vitro. IFN-α significantly decreased colony formation of all three cell lines, and in contrast to normal cells, induced apoptosis in JY and BL-41 and excessive necrosis in HHV-8 infected BCBL-1 cells. FcγRII was upregulated both in normal and in leukemic B cells as indicated by an increase both in the proportion of CD32-positive cells and the mean fluorescence intensity. From our results it seems that antiproliferative, apoptotic and differentiative effects of IFN-α are interrelated but distinct cellular events, which are differentially regulated in normal, leukemic and virus-infected cells of the B-cell lineage.",
keywords = "Apoptosis, B cells, Colony formation, Interferon-alpha, Type II Fcγ receptor",
author = "Istv{\'a}n Szegedi and C. Kiss and {\'E}va Kar{\'a}szi and G. V{\'a}mosi and J. Sz{\"o}llősi and P{\'e}ter Kov{\'a}cs and Ilona Benko",
year = "2006",
language = "English",
volume = "12",
pages = "159--163",
journal = "Pathology and Oncology Research",
issn = "1219-4956",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Differential regulation of umbilical cord blood and leukemic B cells by interferon-alpha (IFN-α)

T2 - Observations in cultured cells

AU - Szegedi, István

AU - Kiss, C.

AU - Karászi, Éva

AU - Vámosi, G.

AU - Szöllősi, J.

AU - Kovács, Péter

AU - Benko, Ilona

PY - 2006

Y1 - 2006

N2 - The exact mechanism of the beneficial therapeutic action of interferon-a (IFN-α) in B-cell-lineage malignancies has not been adequately explained. Here we report on the differential effect of IFN-α2b on non-malignant 6 cells of umbilical cord blood and leukemic B-cell lines JY, BL-41 and BCBL-1. Leukemic cell proliferation was characterized by colony assay, whereas apoptosis was investigated by flow cytometry of propidium iodide-stained cells. The degree of differentiation was evaluated by measuring the expression level of Fcγ receptor-II (FcγRII) labeled with anti-CD32-FITC monoclonal antibody using flow cytometry. IFN-α protected umbilical cord blood CD19-positive B lymphocytes from apoptotic cell death in vitro. IFN-α significantly decreased colony formation of all three cell lines, and in contrast to normal cells, induced apoptosis in JY and BL-41 and excessive necrosis in HHV-8 infected BCBL-1 cells. FcγRII was upregulated both in normal and in leukemic B cells as indicated by an increase both in the proportion of CD32-positive cells and the mean fluorescence intensity. From our results it seems that antiproliferative, apoptotic and differentiative effects of IFN-α are interrelated but distinct cellular events, which are differentially regulated in normal, leukemic and virus-infected cells of the B-cell lineage.

AB - The exact mechanism of the beneficial therapeutic action of interferon-a (IFN-α) in B-cell-lineage malignancies has not been adequately explained. Here we report on the differential effect of IFN-α2b on non-malignant 6 cells of umbilical cord blood and leukemic B-cell lines JY, BL-41 and BCBL-1. Leukemic cell proliferation was characterized by colony assay, whereas apoptosis was investigated by flow cytometry of propidium iodide-stained cells. The degree of differentiation was evaluated by measuring the expression level of Fcγ receptor-II (FcγRII) labeled with anti-CD32-FITC monoclonal antibody using flow cytometry. IFN-α protected umbilical cord blood CD19-positive B lymphocytes from apoptotic cell death in vitro. IFN-α significantly decreased colony formation of all three cell lines, and in contrast to normal cells, induced apoptosis in JY and BL-41 and excessive necrosis in HHV-8 infected BCBL-1 cells. FcγRII was upregulated both in normal and in leukemic B cells as indicated by an increase both in the proportion of CD32-positive cells and the mean fluorescence intensity. From our results it seems that antiproliferative, apoptotic and differentiative effects of IFN-α are interrelated but distinct cellular events, which are differentially regulated in normal, leukemic and virus-infected cells of the B-cell lineage.

KW - Apoptosis

KW - B cells

KW - Colony formation

KW - Interferon-alpha

KW - Type II Fcγ receptor

UR - http://www.scopus.com/inward/record.url?scp=33750889833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750889833&partnerID=8YFLogxK

M3 - Article

C2 - 16998596

AN - SCOPUS:33750889833

VL - 12

SP - 159

EP - 163

JO - Pathology and Oncology Research

JF - Pathology and Oncology Research

SN - 1219-4956

IS - 3

ER -