Differential regulation of the two metallothionein genes in common carp

E. Hermesz, Anett P. Gazdag, K. Said Ali, J. Nemcsók, Magdolna Ábrahám

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.

Original languageEnglish
Pages (from-to)343-350
Number of pages8
JournalActa Biologica Hungarica
Volume53
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Carps
metallothionein
Metallothionein
Cyprinus carpio
Genes
gene
Liver
genes
Kidney
kidneys
liver
Messenger RNA
Arsenic
regulation
arsenic
Copper
carp
Protein Isoforms
Metals
Tissue

Keywords

  • Carp
  • Gene expression
  • Metal treatment
  • Metallothionein mRNAs
  • RT-PCR

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Differential regulation of the two metallothionein genes in common carp. / Hermesz, E.; Gazdag, Anett P.; Said Ali, K.; Nemcsók, J.; Ábrahám, Magdolna.

In: Acta Biologica Hungarica, Vol. 53, No. 3, 2002, p. 343-350.

Research output: Contribution to journalArticle

Hermesz, E. ; Gazdag, Anett P. ; Said Ali, K. ; Nemcsók, J. ; Ábrahám, Magdolna. / Differential regulation of the two metallothionein genes in common carp. In: Acta Biologica Hungarica. 2002 ; Vol. 53, No. 3. pp. 343-350.
@article{63777dd1789349fe84001ce569e3c76f,
title = "Differential regulation of the two metallothionein genes in common carp",
abstract = "The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.",
keywords = "Carp, Gene expression, Metal treatment, Metallothionein mRNAs, RT-PCR",
author = "E. Hermesz and Gazdag, {Anett P.} and {Said Ali}, K. and J. Nemcs{\'o}k and Magdolna {\'A}brah{\'a}m",
year = "2002",
doi = "10.1556/ABiol.53.2002.3.11",
language = "English",
volume = "53",
pages = "343--350",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Differential regulation of the two metallothionein genes in common carp

AU - Hermesz, E.

AU - Gazdag, Anett P.

AU - Said Ali, K.

AU - Nemcsók, J.

AU - Ábrahám, Magdolna

PY - 2002

Y1 - 2002

N2 - The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.

AB - The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.

KW - Carp

KW - Gene expression

KW - Metal treatment

KW - Metallothionein mRNAs

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=0036024924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036024924&partnerID=8YFLogxK

U2 - 10.1556/ABiol.53.2002.3.11

DO - 10.1556/ABiol.53.2002.3.11

M3 - Article

VL - 53

SP - 343

EP - 350

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 3

ER -