Differential regulation of major histocompatibility complex class II expression and nitric oxide release by β-amyloid in rat astrocyte and microglia

Tamas Pazmany, Laszlo Mechtler, Thomas B. Tomasi, J. Kósa, Andrea Turoczi, Zoltan Urbanyi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Astrocytes and microglial cells were examined for expression of two immunologically important molecules, major histocompatibility complex class II (MHC-II) and nitric oxide (NO) following treatment with IFN-γ and β- amyloid (βA) peptides, βA(1-42) and βA(25-35). IFN-γ is a potent inducer of both MHC-II gene expression and NO production. The induction of MHC-II was inhibited by both βA peptides in astrocytes but they had little or no effect in microglia, βA peptides had no effect on NO release in astrocytes but on microglia βA(1-42) synergistically induced NO release with IFN-γ. Transient transfection of astrocytes with 5' deletional mutants of MHC-II IAα promoter linked to the chloramphenicol acetyl transferase reporter gene (IAα-CAT), demonstrated that βA acts at the transcriptional level to downregulate IFN- γ induced MHC-II gene expression in astrocytes. In previous studies, the induction of MHC-II on glial cells were suggested to be involved in the pathogenesis of neurodegenerative diseases and MHC-II+ microglial cells were observed at much higher frequency than astrocytes. This study provides information on the regulation of the MHC-II gene expression in astrocytes and in microglial cells by βA and this pathway may be critically involved in the immune/inflammatory regulation within the central nervous system.

Original languageEnglish
Pages (from-to)213-223
Number of pages11
JournalBrain Research
Volume835
Issue number2
DOIs
Publication statusPublished - Jul 24 1999

Fingerprint

Microglia
Major Histocompatibility Complex
Amyloid
Astrocytes
Nitric Oxide
MHC Class II Genes
Gene Expression
Peptides
Chloramphenicol
Transferases
Reporter Genes
Neuroglia
Neurodegenerative Diseases
Transfection
Down-Regulation
Central Nervous System

Keywords

  • β-amyloid
  • Alzheimer's disease
  • Astrocyte
  • MHC-II
  • Microglia
  • NO

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential regulation of major histocompatibility complex class II expression and nitric oxide release by β-amyloid in rat astrocyte and microglia. / Pazmany, Tamas; Mechtler, Laszlo; Tomasi, Thomas B.; Kósa, J.; Turoczi, Andrea; Urbanyi, Zoltan.

In: Brain Research, Vol. 835, No. 2, 24.07.1999, p. 213-223.

Research output: Contribution to journalArticle

Pazmany, Tamas ; Mechtler, Laszlo ; Tomasi, Thomas B. ; Kósa, J. ; Turoczi, Andrea ; Urbanyi, Zoltan. / Differential regulation of major histocompatibility complex class II expression and nitric oxide release by β-amyloid in rat astrocyte and microglia. In: Brain Research. 1999 ; Vol. 835, No. 2. pp. 213-223.
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