Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor

L. Hunyady, Albert J. Baukal, Z. Gáborik, Jesus A. Olivares-Reyes, Márta Bor, Márta Szaszák, Robert Lodge, Kevin J. Catt, Tamas Balla

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Agonist-induced endocytosis and processing of the G protein-coupled AT1 angiotensin II (Ang II) receptor (AT1R) was studied in HEK 293 cells expressing green fluorescent protein (GFP)- or hemagglutinin epitope-tagged forms of the receptor. After stimulation with Ang II, the receptor and its ligand colocalized with Rab5-GFP and Rab4-GFP in early endosomes, and subsequently with Rab11-GFP in pericentriolar recycling endosomes. Inhibition of phosphatidylinositol (PI) 3-kinase by wortmannin (WT) or LY294002 caused the formation of large endosomal vesicles of heterogeneous Rab composition, containing the ligand-receptor complex in their limiting membranes and in small associated vesicular structures. In contrast to Alexa®-transferrin, which was mainly found in small vesicles associated with the outside of large vesicles in WT-treated cells, rhodamine-Ang Il was also segregated into small internal vesicles. In cells labeled with 125I-Ang II, WT treatment did not impair the rate of receptor endocytosis, but significantly reduced the initial phase of receptor recycling without affecting its slow component. Similarly, WT inhibited the early, but not the slow, component of the recovery of AT1 R at the cell surface after termination of Ang II stimulation. These data indicate that internalized AT1 receptors are processed via vesicles that resemble multivesicular bodies, and recycle to the cell surface by a rapid PI 3-kinase-dependent recycling route, as well as by a slower pathway that is less sensitive to PI 3-kinase inhibitors.

Original languageEnglish
Pages (from-to)1211-1222
Number of pages12
JournalJournal of Cell Biology
Volume157
Issue number7
DOIs
Publication statusPublished - Jun 24 2002

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Phosphatidylinositol 3-Kinase
Angiotensin Type 1 Receptor
Recycling
Green Fluorescent Proteins
Angiotensin Receptors
Endosomes
Endocytosis
Angiotensin II
Ligands
Multivesicular Bodies
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Rhodamines
HEK293 Cells
Hemagglutinins
Transferrin
GTP-Binding Proteins
Epitopes
Membranes
wortmannin

Keywords

  • Angiotensin II receptor recycling
  • Endosomal sorting
  • G protein-coupled receptor
  • PI 3-kinase
  • Rab proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor. / Hunyady, L.; Baukal, Albert J.; Gáborik, Z.; Olivares-Reyes, Jesus A.; Bor, Márta; Szaszák, Márta; Lodge, Robert; Catt, Kevin J.; Balla, Tamas.

In: Journal of Cell Biology, Vol. 157, No. 7, 24.06.2002, p. 1211-1222.

Research output: Contribution to journalArticle

Hunyady, L. ; Baukal, Albert J. ; Gáborik, Z. ; Olivares-Reyes, Jesus A. ; Bor, Márta ; Szaszák, Márta ; Lodge, Robert ; Catt, Kevin J. ; Balla, Tamas. / Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor. In: Journal of Cell Biology. 2002 ; Vol. 157, No. 7. pp. 1211-1222.
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