Differential patterns of interaction between HIV type 1 and HTLV type I in monocyte-derived macrophages cultured in vitro: Implications for in vivo coinfection with HIV type 1 and HTLV type i

J. Szabó, Zoltán Beck, Éva Csomán, Xiangdong Liu, István Andirkó, Jolán Kiss, Attila Bácsi, Peter Ebbesen, F. Tóth

Research output: Contribution to journalArticle

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Abstract

The interaction between human immunodeficiency virus type 1 (HIV-1) and human T cell leukemia-lymphoma virus type I (HTLV-I) has generated substantial interest. However, there is disagreement on the in vivo consequences of the double infection. We investigated the interactions between HIV-1 and HTLV-I in monocyte-derived macrophages cultured in vitro. For study, the T cell-tropic strain IIB and the macrophage-tropic strain Ada- M of HIV-1 were used. The HTLV-I was prepared from the supernatants of the virus-producing MT-2 cell line. We found that coinfection of macrophages with T cell-tropic HIV-1 and HTLV-I significantly enhanced HIV-1 replication, whereas double infection of the cells with macrophage-tropic HIV-1 and HTLV-I resulted in marked upregulation of HTLV-I production. Stimulatory interactions between HIV-1 and HTLV-I were mediated by their trans-acting proteins. Results of study on nuclear translocation of proviral DNA showed that the tax gene product of HTLV-I was able to facilitate the nuclear import of the reverse-transcribed HIV-1(IIIB) DNA. In contrast, the HIV-1 Tat protein did not increase the intranuclear trafficking of HTLV-I DNA, which suggests another mechanism for HTLV-I enhancement by the tat gene product. In conclusion, this study provides possible mechanisms whereby coinfection of an individual with HIV-1 and HTLV-I may influence the clinical outcome of double infection.

Original languageEnglish
Pages (from-to)1653-1666
Number of pages14
JournalAIDS Research and Human Retroviruses
Volume15
Issue number18
DOIs
Publication statusPublished - Dec 10 1999

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Deltaretrovirus
Human T-lymphotropic virus 1
Coinfection
HIV-1
Macrophages
tat Gene Products
DNA
In Vitro Techniques
Infection
tax Gene Products
T-Lymphocytes
Cell Nucleus Active Transport
Virus Replication
Up-Regulation

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Differential patterns of interaction between HIV type 1 and HTLV type I in monocyte-derived macrophages cultured in vitro : Implications for in vivo coinfection with HIV type 1 and HTLV type i. / Szabó, J.; Beck, Zoltán; Csomán, Éva; Liu, Xiangdong; Andirkó, István; Kiss, Jolán; Bácsi, Attila; Ebbesen, Peter; Tóth, F.

In: AIDS Research and Human Retroviruses, Vol. 15, No. 18, 10.12.1999, p. 1653-1666.

Research output: Contribution to journalArticle

Szabó, J. ; Beck, Zoltán ; Csomán, Éva ; Liu, Xiangdong ; Andirkó, István ; Kiss, Jolán ; Bácsi, Attila ; Ebbesen, Peter ; Tóth, F. / Differential patterns of interaction between HIV type 1 and HTLV type I in monocyte-derived macrophages cultured in vitro : Implications for in vivo coinfection with HIV type 1 and HTLV type i. In: AIDS Research and Human Retroviruses. 1999 ; Vol. 15, No. 18. pp. 1653-1666.
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