Differential patterns of human cytomegalovirus gene expression in various T-cell lines carrying human T-cell leukemia-lymphoma virus type I: Role of tax-activated cellular transcription factors

Zoltán Beck, Attila Bácsi, Xiangdong Liu, Peter Ebbesen, István Andirkó, Eszter Csoma, József Kónya, Etelka Nagy, Ferenc D. Tóth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Replication of human cytomegalovirus (HCMV) was investigated in various T-cell lines expressing the tax gene product of human T-cell leukemia-lymphoma virus type I (HTLV-I). Differential patterns of HCMV replication were found in HTLV-I-carrying cell lines. HCMV gene expression was restricted to the immediate-early genes in MT-2 and MT-4 cells, whereas full replication cycle of the virus was observed in C8166-45 cells. Productive HCMV infection induced a cytopathic effect resulting in the lysis of infected cells. The results of electrophoretic mobility shift assay (EMSA) showed high levels of NF-κB-, CREB/ATF-1-, and SRF-specific DNA binding activity in all Tax-positive cell lines. In contrast, SP1 activity could be detected only in C8166-45 cells. Using an inducible system (Jurkat cell line JPX-9), a dramatic increase in NF-κB, CREB/ATF-1, SRF, and SP1 binding activity, as well as productive HCMV infection, were observed upon Tax expression. Overexpression of SP1 in MT-2 and MT-4 cells converted HCMV infection from an abortive to a productive one. These data suggest that the stimulatory effect of Tax protein on HCMV in T cells is accomplished through at least five host-related transcription factor pathways. The results of this study provide possible mechanisms whereby HCMV infections might imply suppression of adult T-cell leukemia.

Original languageEnglish
Pages (from-to)94-104
Number of pages11
JournalJournal of Medical Virology
Volume71
Issue number1
DOIs
Publication statusPublished - Sep 1 2003

Keywords

  • HCMV
  • HTLV-I
  • Replication
  • T-cell lines
  • Tax

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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