Differential ontogeny of divalent cation effects on rat brain delta-, mu-, and kappa-opioid receptor binding.

G. M. Oetting, M. Szücs, C. J. Coscia

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Abstract

The effect of the divalent cations, Mn2+, Mg2+ and Ca2+ on rat forebrain delta-, mu- and kappa-receptor binding was examined during postnatal development. It was found that delta-receptor binding, assessed with [3H]D-Ala2-D-Leu5-enkephalin ([3H]DADLE) (+ 10 nM D-Ala2- MePhe4-Gly-ol5-enkephalin (DAMGE)), was stimulated by the 3 cations in a dose- and developmental time-dependent manner. delta-Binding was most sensitive to the cations during the first week postnatal, prior to the appearance of high-affinity delta-binding. In contrast, inhibition of mu-receptor binding ([3H]DAMGE) by divalent cations appeared early in development and remained constant throughout the postnatal period. Divalent cation inhibition of kappa-binding ([3H]ethylketocyclazocine ([3H]EKC) + 100 nM DAMGE and 100 nM DADLE) appeared after the second week postnatal. These results demonstrate that the characteristics and postnatal development of divalent cation modulation of mu-, delta- and kappa-binding is distinctly different. Thus, the neonate may be a good model system to examine the binding properties and functions of delta- and kappa-receptor subtypes.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalBrain Research
Volume428
Issue number2
Publication statusPublished - Feb 1987

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kappa Opioid Receptor
mu Opioid Receptor
Divalent Cations
Enkephalins
delta Opioid Receptor
Brain
Cations
Leucine-2-Alanine Enkephalin
Ethylketocyclazocine
Prosencephalon

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential ontogeny of divalent cation effects on rat brain delta-, mu-, and kappa-opioid receptor binding. / Oetting, G. M.; Szücs, M.; Coscia, C. J.

In: Brain Research, Vol. 428, No. 2, 02.1987, p. 223-227.

Research output: Contribution to journalArticle

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