Differential ontogeny of divalent cation effects on rat brain δ-, μ-, and χ-opioid receptor binding

Gregory M. Oetting, Maria Szücs, Carmine J. Coscia

Research output: Contribution to journalArticle

1 Citation (Scopus)


The effect of the divalent cations, Mn2+, Mg2+ and Ca2+ on rat forebrain δ-, μ- and χ-receptor binding was examined during postnatal development. It was found that δ-receptor binding, assessed with [3H]d-Ala2-d-Leu5-enkephalin ([3H]DADLE) (+10 nM d-Ala2-MePhe4-Gly-ol5-enkephalin (DAMGE)), was stimulated by the 3 cations in a dose- and developmental time-dependent manner. δ-Binding was most sensitive to the cations during the first week postnatal, prior to the appearance of high-affinity δ-binding. In contrast, inhibition of μ-receptor binding ([3H]DAMGE) by divalent cations appeared early in development and remained constant throughout the postnatal period. Divalent cation inhibition of χ-binding ([3H]ethylketocyclazocine ([3H]EKC) +100 nM DAMGE and 100 nM DADLE) appeared after the second week postnatal. These results demonstrate that the characteristics and postnatal development of divalent cation modulation of μ-, δ- and χ-binding is distinctly different. Thus, the neonate may be a good model system to examine the binding properties and functions of δ- and χ-receptor subtypes.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalDevelopmental Brain Research
Issue number2
Publication statusPublished - Feb 1987


  • Divalent cation
  • Opioid receptor
  • Postnatal development
  • Rat forebrain

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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