Differential mechanisms involved in the effect of nicotinic agonists DMPP and lobeline to release [3H]5-HT from rat hippocampal slices

B. Lendvai, H. Sershen, A. Lajtha, E. Santha, M. Baranyi, E. Vízi

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

In the present study we investigated the effect of different nicotinic agonists (dimethylphenylpiperazinium-iodide (DMPP), (-)nicotine, cytisine, (-)-lobeline, and (-)epibatidine) and antagonists (mecamylamine and dihydro-β-erythroidine) on the release of [3H]5-HT from hippocampal slices. The nicotinic agonists DMPP and lobeline and electrical field stimulation, released [3H]5-HT from the hippocampus; other nicotinic agonists, such as (-)-nicotine, cytisine, and (-)-epibatidine had no effect. Unlike lobeline-induced release of [3H]5-HT, the effect of DMPP (10 and 40 μM) was antagonized by mecamylamine (20 and 10 μM). The effect of DMPP was [Ca2+](o)-independent. In experiments carried out at 7°C, i.e. the membrane carrier proteins are inhibited and the release by lobeline was abolished while the DMPP-induced release of 5-HT was rather potentiated. It is proposed that the effect of DMPP and lobeline, to enhance the release of [3H]5-HT from the hippocampus, was mediated by two different mechanisms. While DMPP-induced 5-HT release can be linked to a non-classical nAChR activation ([Ca2+](o)-independence), the effect of lobeline was likely mediated by uptake carriers.

Original languageEnglish
Pages (from-to)1769-1777
Number of pages9
JournalNeuropharmacology
Volume35
Issue number12
DOIs
Publication statusPublished - Dec 1996

Fingerprint

Dimethylphenylpiperazinium Iodide
Lobeline
Nicotinic Agonists
Serotonin
epibatidine
Mecamylamine
Nicotine
Hippocampus
Electric Stimulation
Carrier Proteins
Membrane Proteins

Keywords

  • DMPP lobeline
  • hippocampus
  • low temperature
  • serotonin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Differential mechanisms involved in the effect of nicotinic agonists DMPP and lobeline to release [3H]5-HT from rat hippocampal slices. / Lendvai, B.; Sershen, H.; Lajtha, A.; Santha, E.; Baranyi, M.; Vízi, E.

In: Neuropharmacology, Vol. 35, No. 12, 12.1996, p. 1769-1777.

Research output: Contribution to journalArticle

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