Differential localization of GABA-dependent and GABA-independent benzodiazepine binding sites within synapses of rat cerebral cortex

J. Kardos, F. Hajós, M. Simonyi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Fractions containing non-junctional membranes (fraction 1) could be separated from those enriched in junctional complexes (fraction 2) by sucrose gradient centrifugation of osmotically disrupted rat cerebral cortex synaptosomes. [3H]flunitrazepam binding sites were found to be homogeneous characterized by Kd = 0.65 nM, Bmax = 13 pM for fraction 1; and Kd = 2 nM, Bmax = 45 pM for fraction 2. GABA-dependent benzodiazepine receptor sites were found to be localized to the junctional complex fraction, while non-junctional membranes showed GABA-independent receptor and acceptor binding sites.

Original languageEnglish
Pages (from-to)355-359
Number of pages5
JournalNeuroscience Letters
Volume48
Issue number3
DOIs
Publication statusPublished - Aug 10 1984

Fingerprint

Benzodiazepines
Cerebral Cortex
Synapses
gamma-Aminobutyric Acid
Binding Sites
Flunitrazepam
GABA Receptors
Membranes
Synaptosomes
GABA-A Receptors
Centrifugation
Sucrose

Keywords

  • benzodiazepine
  • cerebral cortex
  • junctional complexes
  • non-junctional membranes
  • rat
  • Ro 15-1788
  • Ro 5-4864
  • γ-aminobutyric acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "Fractions containing non-junctional membranes (fraction 1) could be separated from those enriched in junctional complexes (fraction 2) by sucrose gradient centrifugation of osmotically disrupted rat cerebral cortex synaptosomes. [3H]flunitrazepam binding sites were found to be homogeneous characterized by Kd = 0.65 nM, Bmax = 13 pM for fraction 1; and Kd = 2 nM, Bmax = 45 pM for fraction 2. GABA-dependent benzodiazepine receptor sites were found to be localized to the junctional complex fraction, while non-junctional membranes showed GABA-independent receptor and acceptor binding sites.",
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AU - Hajós, F.

AU - Simonyi, M.

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