Differential interferon production in human first and third trimester trophoblast cultures stimulated with viruses

G. Aboagye-Mathiesen, F. Tóth, P. M. Petersen, A. Gildberg, N. Norskov-Lauritsen, V. Zachar, P. Ebbesen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Stimulation of human placental first and third trimester trophoblast and syncytiotrophoblast cultures with viruses [Newcastle Disease Virus (NDV) and Sendai virus] led to a high interferon (IFN) production. The magnitude of the production was dependent on the gestational age of the trophoblast type of inducer and the stage of differentiation of the trophoblast. The data obtained indicated that the first trimester trophoblast cultures produced five to sixfold more IFN than the third trimester trophoblast on per cell basis whereas syncytiotrophoblast at term produced twice as much IFN than the mononuclear term trophoblast when stimulated with the viruses. NDV and Sendai virus produced different levels and composition of IFN-α and -β in both first and third trimester trophoblast and syncytiotrophoblast cultures. Purification of the virus-induced trophoblast interferons (tro-IFNs) by tandem high-performance affinity chromatography resulted in specific activities between 0.7 and 2.7 x 108 IU/mg of protein when assayed on human amniotic WISH cells. The tro-IFN-α protected both human and bovine MDBK cells from virus infection whereas the tro-IFN-β protected only the human cell lines tested. The possible roles of the tro-IFNs are discussed in light of the observed differences in trophoblast IFN response.

Original languageEnglish
Pages (from-to)225-234
Number of pages10
JournalPlacenta
Volume14
Issue number2
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Fingerprint Dive into the research topics of 'Differential interferon production in human first and third trimester trophoblast cultures stimulated with viruses'. Together they form a unique fingerprint.

  • Cite this

    Aboagye-Mathiesen, G., Tóth, F., Petersen, P. M., Gildberg, A., Norskov-Lauritsen, N., Zachar, V., & Ebbesen, P. (1993). Differential interferon production in human first and third trimester trophoblast cultures stimulated with viruses. Placenta, 14(2), 225-234.