Differential inhibition of single and cluster type tumor cell migration

Revekka Harisi, I. Kenessey, Julia N. Olah, Ferenc Timar, Istvan Babo, Gabor Pogany, S. Paku, A. Jeney

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

For the control of tumor metastasis it is important to identify chemical compounds with antimigratory potency. Agents acting against single cell and cluster type migration are necessary for successful antimetastatic therapy. In the present study, the migration of HT-1080 fibrosarcoma cells and OSCORT osteosarcoma cells was compared in a Boyden chamber and in an extracellular matrix (ECM)-based three-dimensional cell culture (3-DCC) model system. The Boyden chamber offers a model of single tumor cell migration, whereas the 3-DCC model system demonstrates invasive growth in the form of a cluster. Since PD98059 (MEK inhibitor) exclusively reduced migration in the 3-DCC model, it may be plausible that the ERK/MAPK signaling pathway is essential for cluster type migration. Interestingly, single cell migration was stimulated upon blocking phosphatidylinositol 3-kinase (PI3K) and also p38-MAPK by treatment with LY294002 and SB203580 respectively. A remarkable reduction of single cell migration was observed following treatment with okadaic acid, a phosphatase 1 (PP1) and 2A (PP2A) inhibitor, which was rather intriguing. This study provided evidence that certain cytotoxic/cytostatic agents at appropriate concentrations were able to preferentially inhibit certain types of migration relative to cell proliferation. Single cell migration was selectively inhibited by taxol at very low subtoxic concentration, whereas 5-hexyl-2'-deoxyuridine (HUdR) exclusively inhibited the cluster type of migration. The borrelidin compound was able to inhibit both types of tumor cell migration, but single tumor cell migration was much less affected. It is interesting that migration was more reduced than proliferation by borrelidin, especially at the advanced growth stage. Taxol is recommended as an agent acting against single cell migration, as well as HUdR and borrelidin as leading compounds for developing antimetastatic drugs against cluster type migration.

Original languageEnglish
Pages (from-to)2981-2985
Number of pages5
JournalAnticancer Research
Volume29
Issue number8
Publication statusPublished - Aug 2009

Fingerprint

Cell Movement
Neoplasms
Paclitaxel
Phosphatidylinositol 3-Kinase
Okadaic Acid
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Fibrosarcoma
Mitogen-Activated Protein Kinase Kinases
Cytotoxins
Cytostatic Agents
p38 Mitogen-Activated Protein Kinases
Osteosarcoma
Growth
Acid Phosphatase
Extracellular Matrix
Cell Culture Techniques
Cell Proliferation
Neoplasm Metastasis
Pharmaceutical Preparations
borrelidin

Keywords

  • 5-hexyl-2′-deoxyuridine
  • Antisense oligonucleotide
  • Borrelidin
  • Boyden chamber
  • Extracellular matrix
  • Migration
  • Signal pathway inhibitors
  • Taxol
  • Three-dimensional cell-culture model

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Harisi, R., Kenessey, I., Olah, J. N., Timar, F., Babo, I., Pogany, G., ... Jeney, A. (2009). Differential inhibition of single and cluster type tumor cell migration. Anticancer Research, 29(8), 2981-2985.

Differential inhibition of single and cluster type tumor cell migration. / Harisi, Revekka; Kenessey, I.; Olah, Julia N.; Timar, Ferenc; Babo, Istvan; Pogany, Gabor; Paku, S.; Jeney, A.

In: Anticancer Research, Vol. 29, No. 8, 08.2009, p. 2981-2985.

Research output: Contribution to journalArticle

Harisi, R, Kenessey, I, Olah, JN, Timar, F, Babo, I, Pogany, G, Paku, S & Jeney, A 2009, 'Differential inhibition of single and cluster type tumor cell migration', Anticancer Research, vol. 29, no. 8, pp. 2981-2985.
Harisi R, Kenessey I, Olah JN, Timar F, Babo I, Pogany G et al. Differential inhibition of single and cluster type tumor cell migration. Anticancer Research. 2009 Aug;29(8):2981-2985.
Harisi, Revekka ; Kenessey, I. ; Olah, Julia N. ; Timar, Ferenc ; Babo, Istvan ; Pogany, Gabor ; Paku, S. ; Jeney, A. / Differential inhibition of single and cluster type tumor cell migration. In: Anticancer Research. 2009 ; Vol. 29, No. 8. pp. 2981-2985.
@article{1e0ba13dc7a94b7499d8c7041f1631b9,
title = "Differential inhibition of single and cluster type tumor cell migration",
abstract = "For the control of tumor metastasis it is important to identify chemical compounds with antimigratory potency. Agents acting against single cell and cluster type migration are necessary for successful antimetastatic therapy. In the present study, the migration of HT-1080 fibrosarcoma cells and OSCORT osteosarcoma cells was compared in a Boyden chamber and in an extracellular matrix (ECM)-based three-dimensional cell culture (3-DCC) model system. The Boyden chamber offers a model of single tumor cell migration, whereas the 3-DCC model system demonstrates invasive growth in the form of a cluster. Since PD98059 (MEK inhibitor) exclusively reduced migration in the 3-DCC model, it may be plausible that the ERK/MAPK signaling pathway is essential for cluster type migration. Interestingly, single cell migration was stimulated upon blocking phosphatidylinositol 3-kinase (PI3K) and also p38-MAPK by treatment with LY294002 and SB203580 respectively. A remarkable reduction of single cell migration was observed following treatment with okadaic acid, a phosphatase 1 (PP1) and 2A (PP2A) inhibitor, which was rather intriguing. This study provided evidence that certain cytotoxic/cytostatic agents at appropriate concentrations were able to preferentially inhibit certain types of migration relative to cell proliferation. Single cell migration was selectively inhibited by taxol at very low subtoxic concentration, whereas 5-hexyl-2'-deoxyuridine (HUdR) exclusively inhibited the cluster type of migration. The borrelidin compound was able to inhibit both types of tumor cell migration, but single tumor cell migration was much less affected. It is interesting that migration was more reduced than proliferation by borrelidin, especially at the advanced growth stage. Taxol is recommended as an agent acting against single cell migration, as well as HUdR and borrelidin as leading compounds for developing antimetastatic drugs against cluster type migration.",
keywords = "5-hexyl-2′-deoxyuridine, Antisense oligonucleotide, Borrelidin, Boyden chamber, Extracellular matrix, Migration, Signal pathway inhibitors, Taxol, Three-dimensional cell-culture model",
author = "Revekka Harisi and I. Kenessey and Olah, {Julia N.} and Ferenc Timar and Istvan Babo and Gabor Pogany and S. Paku and A. Jeney",
year = "2009",
month = "8",
language = "English",
volume = "29",
pages = "2981--2985",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "8",

}

TY - JOUR

T1 - Differential inhibition of single and cluster type tumor cell migration

AU - Harisi, Revekka

AU - Kenessey, I.

AU - Olah, Julia N.

AU - Timar, Ferenc

AU - Babo, Istvan

AU - Pogany, Gabor

AU - Paku, S.

AU - Jeney, A.

PY - 2009/8

Y1 - 2009/8

N2 - For the control of tumor metastasis it is important to identify chemical compounds with antimigratory potency. Agents acting against single cell and cluster type migration are necessary for successful antimetastatic therapy. In the present study, the migration of HT-1080 fibrosarcoma cells and OSCORT osteosarcoma cells was compared in a Boyden chamber and in an extracellular matrix (ECM)-based three-dimensional cell culture (3-DCC) model system. The Boyden chamber offers a model of single tumor cell migration, whereas the 3-DCC model system demonstrates invasive growth in the form of a cluster. Since PD98059 (MEK inhibitor) exclusively reduced migration in the 3-DCC model, it may be plausible that the ERK/MAPK signaling pathway is essential for cluster type migration. Interestingly, single cell migration was stimulated upon blocking phosphatidylinositol 3-kinase (PI3K) and also p38-MAPK by treatment with LY294002 and SB203580 respectively. A remarkable reduction of single cell migration was observed following treatment with okadaic acid, a phosphatase 1 (PP1) and 2A (PP2A) inhibitor, which was rather intriguing. This study provided evidence that certain cytotoxic/cytostatic agents at appropriate concentrations were able to preferentially inhibit certain types of migration relative to cell proliferation. Single cell migration was selectively inhibited by taxol at very low subtoxic concentration, whereas 5-hexyl-2'-deoxyuridine (HUdR) exclusively inhibited the cluster type of migration. The borrelidin compound was able to inhibit both types of tumor cell migration, but single tumor cell migration was much less affected. It is interesting that migration was more reduced than proliferation by borrelidin, especially at the advanced growth stage. Taxol is recommended as an agent acting against single cell migration, as well as HUdR and borrelidin as leading compounds for developing antimetastatic drugs against cluster type migration.

AB - For the control of tumor metastasis it is important to identify chemical compounds with antimigratory potency. Agents acting against single cell and cluster type migration are necessary for successful antimetastatic therapy. In the present study, the migration of HT-1080 fibrosarcoma cells and OSCORT osteosarcoma cells was compared in a Boyden chamber and in an extracellular matrix (ECM)-based three-dimensional cell culture (3-DCC) model system. The Boyden chamber offers a model of single tumor cell migration, whereas the 3-DCC model system demonstrates invasive growth in the form of a cluster. Since PD98059 (MEK inhibitor) exclusively reduced migration in the 3-DCC model, it may be plausible that the ERK/MAPK signaling pathway is essential for cluster type migration. Interestingly, single cell migration was stimulated upon blocking phosphatidylinositol 3-kinase (PI3K) and also p38-MAPK by treatment with LY294002 and SB203580 respectively. A remarkable reduction of single cell migration was observed following treatment with okadaic acid, a phosphatase 1 (PP1) and 2A (PP2A) inhibitor, which was rather intriguing. This study provided evidence that certain cytotoxic/cytostatic agents at appropriate concentrations were able to preferentially inhibit certain types of migration relative to cell proliferation. Single cell migration was selectively inhibited by taxol at very low subtoxic concentration, whereas 5-hexyl-2'-deoxyuridine (HUdR) exclusively inhibited the cluster type of migration. The borrelidin compound was able to inhibit both types of tumor cell migration, but single tumor cell migration was much less affected. It is interesting that migration was more reduced than proliferation by borrelidin, especially at the advanced growth stage. Taxol is recommended as an agent acting against single cell migration, as well as HUdR and borrelidin as leading compounds for developing antimetastatic drugs against cluster type migration.

KW - 5-hexyl-2′-deoxyuridine

KW - Antisense oligonucleotide

KW - Borrelidin

KW - Boyden chamber

KW - Extracellular matrix

KW - Migration

KW - Signal pathway inhibitors

KW - Taxol

KW - Three-dimensional cell-culture model

UR - http://www.scopus.com/inward/record.url?scp=68549115310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68549115310&partnerID=8YFLogxK

M3 - Article

VL - 29

SP - 2981

EP - 2985

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 8

ER -