Differential gene expression analysis by micro-preparative capillary gel electrophoresis

A. Guttman, Liang Shi, Julia Khandurina, Xun Wang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Differential display analysis by cDNA fractionation, collection of differentially expressed fractions of interests and their downstream characterization is demonstrated. cDNA pools from two strains of Cochliobolus heterostrophus fungus were generated by specific restriction digestion and selective ligation. Micropreparative separation and isolation of differentially expressed transcript representatives were accomplished by high-performance capillary gel electrophoresis. The collected individual DNA molecules were polymerase chain reaction amplified and sequenced to create expressed sequence tags for the genes of interests. High resolving power and sensitivity of capillary gel electrophoresis enabled fast and automated processing of minute amounts of cDNA samples with high precision.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalJournal of Chromatography A
Volume1014
Issue number1-2
DOIs
Publication statusPublished - Oct 3 2003

Fingerprint

Capillary Electrophoresis
Electrophoresis
Gene expression
Complementary DNA
Gels
Gene Expression
Ascomycota
Polymerase chain reaction
Expressed Sequence Tags
Optical resolving power
Fractionation
Fungi
Ligation
Digestion
Genes
Display devices
Polymerase Chain Reaction
Molecules
DNA
Processing

Keywords

  • Cochliobolus heterostrophus
  • DNA
  • Genes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Differential gene expression analysis by micro-preparative capillary gel electrophoresis. / Guttman, A.; Shi, Liang; Khandurina, Julia; Wang, Xun.

In: Journal of Chromatography A, Vol. 1014, No. 1-2, 03.10.2003, p. 29-35.

Research output: Contribution to journalArticle

Guttman, A. ; Shi, Liang ; Khandurina, Julia ; Wang, Xun. / Differential gene expression analysis by micro-preparative capillary gel electrophoresis. In: Journal of Chromatography A. 2003 ; Vol. 1014, No. 1-2. pp. 29-35.
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