Differential expressions of protein kinase C isozymes during proliferation and differentiation of human skeletal muscle cells in vitro

Judit Boczán, Sándor Boros, Ferenc Mechler, László Kovács, T. Bíró

Research output: Contribution to journalArticle

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Abstract

The mechanism of skeletal muscle regeneration in vivo can be well modeled in vitro by culturing skeletal muscle cells. In these cultures mononuclear satellite cells fuse to form polynuclear myotubes by proliferation and differentiation. The aim of this study was to determine how the different protein kinase C (PKC) isozymes were expressed during differentiation of human skeletal muscle in vitro. The expressions of desmin, used as a muscle-specific intermediate filament protein marker of differentiation, and of different PKC isozymes were detected by single and double immunohistochemical labeling, and by Western blot analysis. In skeletal muscle cells we could identify five PKC isozymes (PKCα, -γ, -η, -θ and -ζ). The expressions of PKCα and -ζ did not change significantly during differentiation; their levels of expression were high in the early immature cells and remained unchanged in later phases. In contrast, the expression levels of PKCγ and -η increased with differentiation. Furthermore, the cellular localization of PKCγ markedly altered during differentiation, with a perinuclear-nuclear to cytoplasmic translocation. The change in the level of expression of PKCθ during differentiation showed different pattern; its expression was high during the early phases, but a decreased immunostaining was detected in the matured, well-differentiated myotubes. We conclude, therefore, that cultured human skeletal muscle cells possess a characteristic PKC isozyme pattern, and that the different phases of differentiation are accompanied by different expression patterns of the various isozymes. These data suggest the possible functional and differential roles of PKC isozymes in human skeletal muscle differentiation.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalActa Neuropathologica
Volume99
Issue number2
Publication statusPublished - Feb 2000

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Muscle Cells
Protein Kinase C
Isoenzymes
Skeletal Muscle
Skeletal Muscle Fibers
In Vitro Techniques
Intermediate Filament Proteins
Desmin
Differentiation Antigens
Regeneration
Western Blotting
Muscles

Keywords

  • Differentiation
  • Human
  • Isozymes
  • Protein kinase C
  • Skeletal muscle

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Differential expressions of protein kinase C isozymes during proliferation and differentiation of human skeletal muscle cells in vitro. / Boczán, Judit; Boros, Sándor; Mechler, Ferenc; Kovács, László; Bíró, T.

In: Acta Neuropathologica, Vol. 99, No. 2, 02.2000, p. 96-104.

Research output: Contribution to journalArticle

Boczán, Judit ; Boros, Sándor ; Mechler, Ferenc ; Kovács, László ; Bíró, T. / Differential expressions of protein kinase C isozymes during proliferation and differentiation of human skeletal muscle cells in vitro. In: Acta Neuropathologica. 2000 ; Vol. 99, No. 2. pp. 96-104.
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