Differential expression of tissue transglutaminase during in vivo apoptosis of thymocytes induced via distinct signalling pathways

Z. Szondy, P. Molnár, Zoltán Nemes, Michael Boyiadzis, Noémi Kedei, R. Tóth, L. Fésüs

Research output: Contribution to journalArticle

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Abstract

A significant increase in the expression and activity of tissue transglutaminase (tTG), one of the effector elements of apoptosis, was observed during involution of thymus elicited by treatment with either anti-CD3 antibody or dexamethasone or by irradiation. The blood plasma concentration of ε(γ-glutamyl)lysine isodipeptide, the end-product of the digestion of transglutaminase cross-linked proteins, was also elevated in each of these cases. tTG was localized in cells of the cortical layer of the thymus and immunofluorescence double staining revealed that the enzyme appeared in the apoptotic cells. None of these observations could be made when apoptosis was induced by fas-receptor stimulation. The lack of tTG activity in fas-stimulated cells was accompanied with a less organized apoptotic morphology. Our data suggest that distinct signalling pathways, which induce apoptosis within the same cell type, can differentially regulate the expression of tTG, and this enzyme may be involved in structural stabilization of the apoptotic cells.

Original languageEnglish
Pages (from-to)307-313
Number of pages7
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
Publication statusPublished - Mar 10 1997

Fingerprint

Thymocytes
Apoptosis
Thymus
Thymus Gland
Cells
CD95 Antigens
Transglutaminases
Enzymes
Dexamethasone
Lysine
Blood
Stabilization
Irradiation
Fluorescent Antibody Technique
Digestion
Anti-Idiotypic Antibodies
Plasmas
transglutaminase 2
Antibodies
Staining and Labeling

Keywords

  • Apoptosis
  • Signal transduction
  • T lymphocyte
  • Tissue transglutaminase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Differential expression of tissue transglutaminase during in vivo apoptosis of thymocytes induced via distinct signalling pathways. / Szondy, Z.; Molnár, P.; Nemes, Zoltán; Boyiadzis, Michael; Kedei, Noémi; Tóth, R.; Fésüs, L.

In: FEBS Letters, Vol. 404, No. 2-3, 10.03.1997, p. 307-313.

Research output: Contribution to journalArticle

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AU - Kedei, Noémi

AU - Tóth, R.

AU - Fésüs, L.

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