Differential expression of the urokinase receptor (CD87) in arthritic and normal synovial tissues

Z. Szekanecz, G. Kenneth Haines, Alisa E. Koch

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aim - To determine whether the urokinase plasminogen activator receptor (u-PAR; CD87) exhibits a possible pathogenic role in rheumatoid and osteoarthritis. Methods - A semiquantitative, indirect immunoperoxidase histochemical analysis was performed on frozen synovial tissue sections. The recently characterised monoclonal antibody 10G7 recognising transfectants bearing u-PAR was used. Synovial tissue was obtained from 10 patients with rheumatoid arthritis, 10 patients with osteoarthritis, and four normal subjects. Results - u-PAR was expressed on 70-90% of synovial tissue lining cells and subsynovial, interstitial macrophages from the arthritis patients, but only on a few myeloid cells from the normal subjects. It was also present on more endothelial cells from the rheumatoid and osteoarthritis patients, than from normal synovial tissue. Conclusions - Plasminogen activators are important in joint destruction underlying arthritis. The up-regulated expression of u-PAR in diseased versus normal synovial tissue suggests a role for this antigen in the inflammatory and angiogenic mechanisms underlying rheumatoid and osteoarthritis.

Original languageEnglish
Pages (from-to)314-319
Number of pages6
JournalJournal of Clinical Pathology
Volume50
Issue number4
Publication statusPublished - 1997

Fingerprint

Urokinase-Type Plasminogen Activator
Arthritis
Osteoarthritis
Urokinase Plasminogen Activator Receptors
Plasminogen Activators
Myeloid Cells
Rheumatoid Arthritis
Endothelial Cells
Joints
Macrophages
Monoclonal Antibodies
Antigens

Keywords

  • CD87
  • Osteoarthritis
  • Rheumatoid arthritis
  • Urokinase receptor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Differential expression of the urokinase receptor (CD87) in arthritic and normal synovial tissues. / Szekanecz, Z.; Haines, G. Kenneth; Koch, Alisa E.

In: Journal of Clinical Pathology, Vol. 50, No. 4, 1997, p. 314-319.

Research output: Contribution to journalArticle

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AB - Aim - To determine whether the urokinase plasminogen activator receptor (u-PAR; CD87) exhibits a possible pathogenic role in rheumatoid and osteoarthritis. Methods - A semiquantitative, indirect immunoperoxidase histochemical analysis was performed on frozen synovial tissue sections. The recently characterised monoclonal antibody 10G7 recognising transfectants bearing u-PAR was used. Synovial tissue was obtained from 10 patients with rheumatoid arthritis, 10 patients with osteoarthritis, and four normal subjects. Results - u-PAR was expressed on 70-90% of synovial tissue lining cells and subsynovial, interstitial macrophages from the arthritis patients, but only on a few myeloid cells from the normal subjects. It was also present on more endothelial cells from the rheumatoid and osteoarthritis patients, than from normal synovial tissue. Conclusions - Plasminogen activators are important in joint destruction underlying arthritis. The up-regulated expression of u-PAR in diseased versus normal synovial tissue suggests a role for this antigen in the inflammatory and angiogenic mechanisms underlying rheumatoid and osteoarthritis.

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