Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues

Isabelle Brun-Heath, Myriam Ermonval, Elodie Chabrol, Jinsong Xiao, M. Palkóvits, Ruth Lyck, Florence Miller, Pierre Olivier Couraud, Etienne Mornet, Caroline Fonta

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. It is present in large amounts in bone in which it plays a role in mineralization but little is known about its function in other tissues. Arguments are accumulating for its involvement in the brain, in particular in view of the neurological symptoms accompanying human TNAP deficiencies. We have previously shown, by histochemistry, alkaline phosphatase (AP) activity in monkey brain vessels and parenchyma in which AP exhibits specific patterns. Here, we clearly attribute this activity to TNAP expression rather than to other APs in primates (human and marmoset) and in rodents (rat and mouse). We have not found any brain-specific transcripts but our data demonstrate that neuronal and endothelial cells exclusively express the bone TNAP transcript in all species tested, except in mouse neurons in which liver TNAP transcripts have also been detected. Moreover, we highlight the developmental regulation of TNAP expression; this also acts during neuronal differentiation. Our study should help to characterize the regulation of the expression of this ectophosphatase in various cell types of the central nervous system.

Original languageEnglish
Pages (from-to)521-536
Number of pages16
JournalCell and Tissue Research
Volume343
Issue number3
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Alkaline Phosphatase
Protein Isoforms
Bone and Bones
Liver
Brain
Callithrix
Primates
Haplorhini
Rodentia
Central Nervous System
Endothelial Cells
Neurons
Enzymes

Keywords

  • Cerebral endothelial cell
  • Ectophosphatase
  • Neuron
  • Primate
  • Rodent

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. / Brun-Heath, Isabelle; Ermonval, Myriam; Chabrol, Elodie; Xiao, Jinsong; Palkóvits, M.; Lyck, Ruth; Miller, Florence; Couraud, Pierre Olivier; Mornet, Etienne; Fonta, Caroline.

In: Cell and Tissue Research, Vol. 343, No. 3, 03.2011, p. 521-536.

Research output: Contribution to journalArticle

Brun-Heath, I, Ermonval, M, Chabrol, E, Xiao, J, Palkóvits, M, Lyck, R, Miller, F, Couraud, PO, Mornet, E & Fonta, C 2011, 'Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues', Cell and Tissue Research, vol. 343, no. 3, pp. 521-536. https://doi.org/10.1007/s00441-010-1111-4
Brun-Heath, Isabelle ; Ermonval, Myriam ; Chabrol, Elodie ; Xiao, Jinsong ; Palkóvits, M. ; Lyck, Ruth ; Miller, Florence ; Couraud, Pierre Olivier ; Mornet, Etienne ; Fonta, Caroline. / Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. In: Cell and Tissue Research. 2011 ; Vol. 343, No. 3. pp. 521-536.
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