Differential expression of GL7 activation antigen on bone marrow B cell subpopulations and peripheral B cells

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

GL7 was originally described as a 35-kDa late activation antigen on mouse T and B cells. GL7 expression has also been demonstrated on thymocytes, germinal center B cells and some neuronal cell types. Flow-cytometry and immunohistochemistry were used to follow changes in the expression of GL7 during B cell development, amongst B cell subpopulations and various anatomical locations. GL7 is expressed as early as the pro-B cell stage and increases up to the pre-B-I stadium. Expression remains high on pre-B-II and on immature B cells, although slightly decreases during maturation. GL7 is almost completely downregulated when IgD appears on the cell surface. On the periphery only a few B cells are positive and these cells are almost exclusively found in the sIgD- germinal center areas of lymph nodes and spleen. The staining pattern of GL7 is very similar to that of PNA in the lymph nodes but in the bone marrow we have found both B220+PNA+GL7- and B220+PNA+GL7+ populations, showing that GL7 and the antigen recognized by PNA are different. After in vitro stimulation, the GL7hi B cell population has also been found to be IgD negative. Functional comparison between in vitro activated and MACS sorted GL7hi and GL7lo/- spleen B cells of immunized mice showed significantly higher specific and total antibody production as well as antigen presenting capacity in the GL7hi population.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalImmunology Letters
Volume78
Issue number2
DOIs
Publication statusPublished - Sep 3 2001

Fingerprint

Bone Marrow Cells
B-Lymphocytes
Immunoglobulin D
B-Lymphoid Precursor Cells
Germinal Center
Blastodisc
Spleen
Lymph Nodes
Population
Antigens
Thymocytes
antigen GL7
Antibody Formation
Flow Cytometry
Down-Regulation
Bone Marrow
Immunohistochemistry
Staining and Labeling
T-Lymphocytes

Keywords

  • B lymphocytes
  • Bone marrow
  • Cellular activation
  • Cellular differentiation
  • Lymph node

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Differential expression of GL7 activation antigen on bone marrow B cell subpopulations and peripheral B cells. / Cervenák, L.; Magyar, A.; Fajka-Boja, R.; László, Glória.

In: Immunology Letters, Vol. 78, No. 2, 03.09.2001, p. 89-96.

Research output: Contribution to journalArticle

@article{911ba706b18348a6ad2f651853bffac9,
title = "Differential expression of GL7 activation antigen on bone marrow B cell subpopulations and peripheral B cells",
abstract = "GL7 was originally described as a 35-kDa late activation antigen on mouse T and B cells. GL7 expression has also been demonstrated on thymocytes, germinal center B cells and some neuronal cell types. Flow-cytometry and immunohistochemistry were used to follow changes in the expression of GL7 during B cell development, amongst B cell subpopulations and various anatomical locations. GL7 is expressed as early as the pro-B cell stage and increases up to the pre-B-I stadium. Expression remains high on pre-B-II and on immature B cells, although slightly decreases during maturation. GL7 is almost completely downregulated when IgD appears on the cell surface. On the periphery only a few B cells are positive and these cells are almost exclusively found in the sIgD- germinal center areas of lymph nodes and spleen. The staining pattern of GL7 is very similar to that of PNA in the lymph nodes but in the bone marrow we have found both B220+PNA+GL7- and B220+PNA+GL7+ populations, showing that GL7 and the antigen recognized by PNA are different. After in vitro stimulation, the GL7hi B cell population has also been found to be IgD negative. Functional comparison between in vitro activated and MACS sorted GL7hi and GL7lo/- spleen B cells of immunized mice showed significantly higher specific and total antibody production as well as antigen presenting capacity in the GL7hi population.",
keywords = "B lymphocytes, Bone marrow, Cellular activation, Cellular differentiation, Lymph node",
author = "L. Cerven{\'a}k and A. Magyar and R. Fajka-Boja and Gl{\'o}ria L{\'a}szl{\'o}",
year = "2001",
month = "9",
day = "3",
doi = "10.1016/S0165-2478(01)00239-5",
language = "English",
volume = "78",
pages = "89--96",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Differential expression of GL7 activation antigen on bone marrow B cell subpopulations and peripheral B cells

AU - Cervenák, L.

AU - Magyar, A.

AU - Fajka-Boja, R.

AU - László, Glória

PY - 2001/9/3

Y1 - 2001/9/3

N2 - GL7 was originally described as a 35-kDa late activation antigen on mouse T and B cells. GL7 expression has also been demonstrated on thymocytes, germinal center B cells and some neuronal cell types. Flow-cytometry and immunohistochemistry were used to follow changes in the expression of GL7 during B cell development, amongst B cell subpopulations and various anatomical locations. GL7 is expressed as early as the pro-B cell stage and increases up to the pre-B-I stadium. Expression remains high on pre-B-II and on immature B cells, although slightly decreases during maturation. GL7 is almost completely downregulated when IgD appears on the cell surface. On the periphery only a few B cells are positive and these cells are almost exclusively found in the sIgD- germinal center areas of lymph nodes and spleen. The staining pattern of GL7 is very similar to that of PNA in the lymph nodes but in the bone marrow we have found both B220+PNA+GL7- and B220+PNA+GL7+ populations, showing that GL7 and the antigen recognized by PNA are different. After in vitro stimulation, the GL7hi B cell population has also been found to be IgD negative. Functional comparison between in vitro activated and MACS sorted GL7hi and GL7lo/- spleen B cells of immunized mice showed significantly higher specific and total antibody production as well as antigen presenting capacity in the GL7hi population.

AB - GL7 was originally described as a 35-kDa late activation antigen on mouse T and B cells. GL7 expression has also been demonstrated on thymocytes, germinal center B cells and some neuronal cell types. Flow-cytometry and immunohistochemistry were used to follow changes in the expression of GL7 during B cell development, amongst B cell subpopulations and various anatomical locations. GL7 is expressed as early as the pro-B cell stage and increases up to the pre-B-I stadium. Expression remains high on pre-B-II and on immature B cells, although slightly decreases during maturation. GL7 is almost completely downregulated when IgD appears on the cell surface. On the periphery only a few B cells are positive and these cells are almost exclusively found in the sIgD- germinal center areas of lymph nodes and spleen. The staining pattern of GL7 is very similar to that of PNA in the lymph nodes but in the bone marrow we have found both B220+PNA+GL7- and B220+PNA+GL7+ populations, showing that GL7 and the antigen recognized by PNA are different. After in vitro stimulation, the GL7hi B cell population has also been found to be IgD negative. Functional comparison between in vitro activated and MACS sorted GL7hi and GL7lo/- spleen B cells of immunized mice showed significantly higher specific and total antibody production as well as antigen presenting capacity in the GL7hi population.

KW - B lymphocytes

KW - Bone marrow

KW - Cellular activation

KW - Cellular differentiation

KW - Lymph node

UR - http://www.scopus.com/inward/record.url?scp=0035801948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035801948&partnerID=8YFLogxK

U2 - 10.1016/S0165-2478(01)00239-5

DO - 10.1016/S0165-2478(01)00239-5

M3 - Article

VL - 78

SP - 89

EP - 96

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 2

ER -