Differential efficacy of vasodilators in hypercholesterolaemic rabbits

Zsuzsanna Rozsa, J. Pataricza, J. Németh, J. Papp

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effects of hypercholesterolaemia on the endothelium-dependent and -independent vascular reactivity of the superior mesenteric artery has been examined in anaesthetized rabbits in-vivo. Rabbits were fed with either standard or cholesterol-enriched diet for 24 weeks. Plasma lipids and changes in the endothelin content of plasma and vascular tissue were measured in the superior mesenteric artery and in the thoracic aorta. The functional severity of atherosclerosis was determined by examining vascular responses in the isolated thoracic aorta. The blood how in the superior mesenteric artery was measured by transit-time flowmetry and drugs were injected through an intra-abdominal aortic catheter. Acetylcholine (5, 10, 20 μg kg-1) elicited dose-dependent, mesenteric vasodilation in normocholesterolaemic rabbits. In hypercholesterolaemic animals the response to acetylcholine was completely abolished and even became a vasoconstriction. Endothelin levels in plasma and in the vascular tissue were significantly elevated in hypercholesterolaemic animals compared with controls. Cromakalim at a dose of 3 μg kg-1, elicited similar mesenteric vasodilation in hypercholesterolaemic and normocholesterolaemic animals. These experiments show that the endothelium-dependent responses of the superior mesenteric artery to acetylcholine are functionally impaired by prolonged hypercholesterolaemia, that this altered vascular reactivity is associated with the elevation of endothelin levels in the circulation and in vascular tissues, and that in hypercholesterolaemia the mesenteric vasodilator effect of the K+-channel opener cromakalim is entirely preserved, suggesting that severe hypercholesterolaemia does not depress the function of ATP-sensitive potassium channels in mesenteric vascular smooth muscle.

Original languageEnglish
Pages (from-to)1035-1044
Number of pages10
JournalJournal of Pharmacy and Pharmacology
Volume50
Issue number9
Publication statusPublished - Sep 1998

Fingerprint

Vasodilator Agents
Blood Vessels
Superior Mesenteric Artery
Rabbits
Hypercholesterolemia
Endothelins
Cromakalim
Acetylcholine
Thoracic Aorta
Vasodilation
Endothelium
KATP Channels
Rheology
Vasoconstriction
Vascular Smooth Muscle
Atherosclerosis
Catheters
Cholesterol
Diet
Lipids

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Differential efficacy of vasodilators in hypercholesterolaemic rabbits. / Rozsa, Zsuzsanna; Pataricza, J.; Németh, J.; Papp, J.

In: Journal of Pharmacy and Pharmacology, Vol. 50, No. 9, 09.1998, p. 1035-1044.

Research output: Contribution to journalArticle

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