Differential effects and interactions of endogenous and horizontally acquired H-NS-like proteins in pathogenic Escherichia coli

Claudia M. Müller, G. Schneider, Ulrich Dobrindt, L. Emődy, Jörg Hacker, Bernt Eric Uhlin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The nucleoid-associated protein H-NS is important for gene regulation in Escherichia coli. We have studied H-NS interaction with StpA and an uncharacterized H-NS-like protein, Hfp, in the uropathogenic E. coli isolate 536 that expresses all three nucleoid-associated proteins. We found distinct interactions of the three proteins at the protein level, resulting in the formation of heteromers, as well as differences in their gene expression at the transcriptional level. Mutants lacking either StpA or Hfp alone did not exhibit a phenotype at 37°C, which is consistent with a low level of expression at that temperature. Expression of the hfp and stpA genes was found to be induced by apparently diametrical conditions, and StpA and Hfp levels could be correlated to modulatory effects on the expression of different H-NS targets, the bgl operon and operons for virulence factors such as fimbriae and capsular polysaccharide. The hns/hfp and hns/stpA double mutants displayed severe growth defects at low and high temperatures respectively. Our findings demonstrated different requirements for the alternative H-NS/Hfp/StpA combinations under these growth conditions. We propose that Hfp and StpA have distinct functions and roles in a dynamic pool of nucleoid-associated proteins that is adapting to requirements in a particular environment.

Original languageEnglish
Pages (from-to)280-293
Number of pages14
JournalMolecular Microbiology
Volume75
Issue number2
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Escherichia coli
Proteins
Operon
Uropathogenic Escherichia coli
Temperature
Virulence Factors
Growth
Genes
Polysaccharides
Phenotype
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Differential effects and interactions of endogenous and horizontally acquired H-NS-like proteins in pathogenic Escherichia coli. / Müller, Claudia M.; Schneider, G.; Dobrindt, Ulrich; Emődy, L.; Hacker, Jörg; Uhlin, Bernt Eric.

In: Molecular Microbiology, Vol. 75, No. 2, 01.2010, p. 280-293.

Research output: Contribution to journalArticle

Müller, Claudia M. ; Schneider, G. ; Dobrindt, Ulrich ; Emődy, L. ; Hacker, Jörg ; Uhlin, Bernt Eric. / Differential effects and interactions of endogenous and horizontally acquired H-NS-like proteins in pathogenic Escherichia coli. In: Molecular Microbiology. 2010 ; Vol. 75, No. 2. pp. 280-293.
@article{6765043671b34c4298a1e5f44be93e82,
title = "Differential effects and interactions of endogenous and horizontally acquired H-NS-like proteins in pathogenic Escherichia coli",
abstract = "The nucleoid-associated protein H-NS is important for gene regulation in Escherichia coli. We have studied H-NS interaction with StpA and an uncharacterized H-NS-like protein, Hfp, in the uropathogenic E. coli isolate 536 that expresses all three nucleoid-associated proteins. We found distinct interactions of the three proteins at the protein level, resulting in the formation of heteromers, as well as differences in their gene expression at the transcriptional level. Mutants lacking either StpA or Hfp alone did not exhibit a phenotype at 37°C, which is consistent with a low level of expression at that temperature. Expression of the hfp and stpA genes was found to be induced by apparently diametrical conditions, and StpA and Hfp levels could be correlated to modulatory effects on the expression of different H-NS targets, the bgl operon and operons for virulence factors such as fimbriae and capsular polysaccharide. The hns/hfp and hns/stpA double mutants displayed severe growth defects at low and high temperatures respectively. Our findings demonstrated different requirements for the alternative H-NS/Hfp/StpA combinations under these growth conditions. We propose that Hfp and StpA have distinct functions and roles in a dynamic pool of nucleoid-associated proteins that is adapting to requirements in a particular environment.",
author = "M{\"u}ller, {Claudia M.} and G. Schneider and Ulrich Dobrindt and L. Emődy and J{\"o}rg Hacker and Uhlin, {Bernt Eric}",
year = "2010",
month = "1",
doi = "10.1111/j.1365-2958.2009.06995.x",
language = "English",
volume = "75",
pages = "280--293",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Differential effects and interactions of endogenous and horizontally acquired H-NS-like proteins in pathogenic Escherichia coli

AU - Müller, Claudia M.

AU - Schneider, G.

AU - Dobrindt, Ulrich

AU - Emődy, L.

AU - Hacker, Jörg

AU - Uhlin, Bernt Eric

PY - 2010/1

Y1 - 2010/1

N2 - The nucleoid-associated protein H-NS is important for gene regulation in Escherichia coli. We have studied H-NS interaction with StpA and an uncharacterized H-NS-like protein, Hfp, in the uropathogenic E. coli isolate 536 that expresses all three nucleoid-associated proteins. We found distinct interactions of the three proteins at the protein level, resulting in the formation of heteromers, as well as differences in their gene expression at the transcriptional level. Mutants lacking either StpA or Hfp alone did not exhibit a phenotype at 37°C, which is consistent with a low level of expression at that temperature. Expression of the hfp and stpA genes was found to be induced by apparently diametrical conditions, and StpA and Hfp levels could be correlated to modulatory effects on the expression of different H-NS targets, the bgl operon and operons for virulence factors such as fimbriae and capsular polysaccharide. The hns/hfp and hns/stpA double mutants displayed severe growth defects at low and high temperatures respectively. Our findings demonstrated different requirements for the alternative H-NS/Hfp/StpA combinations under these growth conditions. We propose that Hfp and StpA have distinct functions and roles in a dynamic pool of nucleoid-associated proteins that is adapting to requirements in a particular environment.

AB - The nucleoid-associated protein H-NS is important for gene regulation in Escherichia coli. We have studied H-NS interaction with StpA and an uncharacterized H-NS-like protein, Hfp, in the uropathogenic E. coli isolate 536 that expresses all three nucleoid-associated proteins. We found distinct interactions of the three proteins at the protein level, resulting in the formation of heteromers, as well as differences in their gene expression at the transcriptional level. Mutants lacking either StpA or Hfp alone did not exhibit a phenotype at 37°C, which is consistent with a low level of expression at that temperature. Expression of the hfp and stpA genes was found to be induced by apparently diametrical conditions, and StpA and Hfp levels could be correlated to modulatory effects on the expression of different H-NS targets, the bgl operon and operons for virulence factors such as fimbriae and capsular polysaccharide. The hns/hfp and hns/stpA double mutants displayed severe growth defects at low and high temperatures respectively. Our findings demonstrated different requirements for the alternative H-NS/Hfp/StpA combinations under these growth conditions. We propose that Hfp and StpA have distinct functions and roles in a dynamic pool of nucleoid-associated proteins that is adapting to requirements in a particular environment.

UR - http://www.scopus.com/inward/record.url?scp=74349105205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74349105205&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2958.2009.06995.x

DO - 10.1111/j.1365-2958.2009.06995.x

M3 - Article

VL - 75

SP - 280

EP - 293

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 2

ER -