Differential effect of nicotinic agonists on the [3H]norepinephrine release from rat hippocampal slices

J. P. Kiss, K. Windisch, K. De Oliveira, E. C P Hennings, A. Mike, B. K. Szász

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The aim of this study was to investigate the mechanisms involved in the effect of nicotinic agonists on the [3H]norepinephrine ([3H]NE) release from rat hippocampal slices. The stimulatory effect of nicotine, cytisine, epibatidine and anatoxin-A was completely blocked by the nicotinic antagonist mecamylamine (10 μM). In contrast, the effect of dimethylphenylpiperazinium (DMPP) was only partially inhibited by mecamylamine but was completely blocked by the NE uptake inhibitor desipramine (DMI, 10 μM). Finally, the effect of lobeline was not affected by mecamylamine and was only partially blocked by DMI. Our data indicate that the majority of nicotinic agonists increase the release of [3H]NE exclusively via stimulation of nicotinic acetylcholine receptors (nAChRs). DMPP, in addition to the stimulation of nAChRs, also evokes a carrier-mediated release. Lobeline has no stimulatory effect on nAChRs, induces a carrier-mediated release and has a further action of unidentified mechanism. Our results suggest that special caution is required for the interpretation of data, when DMPP or lobeline are used as nicotinic agonists.

Original languageEnglish
Pages (from-to)943-950
Number of pages8
JournalNeurochemical Research
Volume26
Issue number8-9
DOIs
Publication statusPublished - 2001

Fingerprint

Dimethylphenylpiperazinium Iodide
Lobeline
Nicotinic Agonists
Mecamylamine
Nicotinic Receptors
Rats
Norepinephrine
epibatidine
Nicotinic Antagonists
Desipramine
Nicotine

Keywords

  • Carrier-mediated release
  • DMPP
  • Hippocampus
  • Lobeline
  • Nicotine
  • Nicotinic acetylcholine receptors
  • Norepinephrine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Differential effect of nicotinic agonists on the [3H]norepinephrine release from rat hippocampal slices. / Kiss, J. P.; Windisch, K.; De Oliveira, K.; Hennings, E. C P; Mike, A.; Szász, B. K.

In: Neurochemical Research, Vol. 26, No. 8-9, 2001, p. 943-950.

Research output: Contribution to journalArticle

Kiss, J. P. ; Windisch, K. ; De Oliveira, K. ; Hennings, E. C P ; Mike, A. ; Szász, B. K. / Differential effect of nicotinic agonists on the [3H]norepinephrine release from rat hippocampal slices. In: Neurochemical Research. 2001 ; Vol. 26, No. 8-9. pp. 943-950.
@article{edd9d26119aa4cfc9a78954fcd6de792,
title = "Differential effect of nicotinic agonists on the [3H]norepinephrine release from rat hippocampal slices",
abstract = "The aim of this study was to investigate the mechanisms involved in the effect of nicotinic agonists on the [3H]norepinephrine ([3H]NE) release from rat hippocampal slices. The stimulatory effect of nicotine, cytisine, epibatidine and anatoxin-A was completely blocked by the nicotinic antagonist mecamylamine (10 μM). In contrast, the effect of dimethylphenylpiperazinium (DMPP) was only partially inhibited by mecamylamine but was completely blocked by the NE uptake inhibitor desipramine (DMI, 10 μM). Finally, the effect of lobeline was not affected by mecamylamine and was only partially blocked by DMI. Our data indicate that the majority of nicotinic agonists increase the release of [3H]NE exclusively via stimulation of nicotinic acetylcholine receptors (nAChRs). DMPP, in addition to the stimulation of nAChRs, also evokes a carrier-mediated release. Lobeline has no stimulatory effect on nAChRs, induces a carrier-mediated release and has a further action of unidentified mechanism. Our results suggest that special caution is required for the interpretation of data, when DMPP or lobeline are used as nicotinic agonists.",
keywords = "Carrier-mediated release, DMPP, Hippocampus, Lobeline, Nicotine, Nicotinic acetylcholine receptors, Norepinephrine",
author = "Kiss, {J. P.} and K. Windisch and {De Oliveira}, K. and Hennings, {E. C P} and A. Mike and Sz{\'a}sz, {B. K.}",
year = "2001",
doi = "10.1023/A:1012384517784",
language = "English",
volume = "26",
pages = "943--950",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "8-9",

}

TY - JOUR

T1 - Differential effect of nicotinic agonists on the [3H]norepinephrine release from rat hippocampal slices

AU - Kiss, J. P.

AU - Windisch, K.

AU - De Oliveira, K.

AU - Hennings, E. C P

AU - Mike, A.

AU - Szász, B. K.

PY - 2001

Y1 - 2001

N2 - The aim of this study was to investigate the mechanisms involved in the effect of nicotinic agonists on the [3H]norepinephrine ([3H]NE) release from rat hippocampal slices. The stimulatory effect of nicotine, cytisine, epibatidine and anatoxin-A was completely blocked by the nicotinic antagonist mecamylamine (10 μM). In contrast, the effect of dimethylphenylpiperazinium (DMPP) was only partially inhibited by mecamylamine but was completely blocked by the NE uptake inhibitor desipramine (DMI, 10 μM). Finally, the effect of lobeline was not affected by mecamylamine and was only partially blocked by DMI. Our data indicate that the majority of nicotinic agonists increase the release of [3H]NE exclusively via stimulation of nicotinic acetylcholine receptors (nAChRs). DMPP, in addition to the stimulation of nAChRs, also evokes a carrier-mediated release. Lobeline has no stimulatory effect on nAChRs, induces a carrier-mediated release and has a further action of unidentified mechanism. Our results suggest that special caution is required for the interpretation of data, when DMPP or lobeline are used as nicotinic agonists.

AB - The aim of this study was to investigate the mechanisms involved in the effect of nicotinic agonists on the [3H]norepinephrine ([3H]NE) release from rat hippocampal slices. The stimulatory effect of nicotine, cytisine, epibatidine and anatoxin-A was completely blocked by the nicotinic antagonist mecamylamine (10 μM). In contrast, the effect of dimethylphenylpiperazinium (DMPP) was only partially inhibited by mecamylamine but was completely blocked by the NE uptake inhibitor desipramine (DMI, 10 μM). Finally, the effect of lobeline was not affected by mecamylamine and was only partially blocked by DMI. Our data indicate that the majority of nicotinic agonists increase the release of [3H]NE exclusively via stimulation of nicotinic acetylcholine receptors (nAChRs). DMPP, in addition to the stimulation of nAChRs, also evokes a carrier-mediated release. Lobeline has no stimulatory effect on nAChRs, induces a carrier-mediated release and has a further action of unidentified mechanism. Our results suggest that special caution is required for the interpretation of data, when DMPP or lobeline are used as nicotinic agonists.

KW - Carrier-mediated release

KW - DMPP

KW - Hippocampus

KW - Lobeline

KW - Nicotine

KW - Nicotinic acetylcholine receptors

KW - Norepinephrine

UR - http://www.scopus.com/inward/record.url?scp=0034753168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034753168&partnerID=8YFLogxK

U2 - 10.1023/A:1012384517784

DO - 10.1023/A:1012384517784

M3 - Article

C2 - 11699946

AN - SCOPUS:0034753168

VL - 26

SP - 943

EP - 950

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 8-9

ER -