Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion

Mariann Gyöngyösi, A. Pósa, Noemi Pavo, Rayyan Hemetsberger, Heda Kvakan, Sabine Steiner-Böker, Zsolt Petrási, Ferenc Manczur, Imre J. Pavo, I. Édes, Johann Wojta, Dietmar Glogar, Kurt Huber

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Effects of ischaemic preconditioning (IP) on the mobilisation and recruitment of haematopoietic (HSCs) and mesenchymal stem (MSC) cells were determined in porcine coronary occlusion/reperfusion. Thirty-three pigs underwent percutaneous occlusion of the left anterior descending coronary artery (LAD) for 90 minutes (min), followed by 120 min reperfusion. IP was performed in 16 of the 33 pigs by two cycles of 5 min balloon occlusion/reperfusion prior to the 90 min occlusion (group IP vs. group C). Peripheral blood and myocardial tissue concentration of bone marrow origin HSCs (characterised by coexpression of CD31+, CD90+, CD45+) and MSCs (characterised by coexpression of CD44+, CD90+, CD45-) were measured by flow cytometry in the early phase of IP. Plasma/serum levels of stem cell mobilisation factors (stromal cell-derived factor-1α [SDF-1α], vascular endothelial growth factor [VEGF], tumour necrosis factor α[TNF-α] and interleukin-8 [IL-8]) were measured. IP led to a significant increase in circulating HSCs as compared with the group C (475 ± 233 vs. 281 ± 264 /μ, p=0.032) in the early phase of IP. In contrast, a rapid and prolonged decrease in level of circulating MSCs was observed in group IP as compared with group C (19 ± 12 vs. 32 ± 17 /μl, p=0.015). The recruitment of HSCs and MSCs in infarct and border zone was significantly greater in IP group, indicating a faster homing of MSCs as compared with the rate of mobilisation. Rapid increase in VEGF, TNF-α and IL-8 levels was induced by IP, which, however, was not correlated with the levels of circulating SCs. In conclusion, IP resulted in differential mobilisation and recruitment of HSCs and MSCs in the early phase of cardioprotection.

Original languageEnglish
Pages (from-to)376-384
Number of pages9
JournalThrombosis and Haemostasis
Volume104
Issue number2
DOIs
Publication statusPublished - Aug 2010

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Ischemic Preconditioning
Myocardial Reperfusion
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Myocardial Ischemia
Swine
Interleukin-8
Vascular Endothelial Growth Factor A
Reperfusion
Tumor Necrosis Factor-alpha
Hematopoietic Stem Cell Mobilization
Balloon Occlusion
Chemokine CXCL12
Stem Cell Factor
Coronary Occlusion
Coronary Vessels
Flow Cytometry
Bone Marrow

Keywords

  • Haematopoietic
  • Ischaemic preconditioning
  • Mesenchymal
  • Stem cell mobilisation
  • Stromal-derived

ASJC Scopus subject areas

  • Hematology

Cite this

Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion. / Gyöngyösi, Mariann; Pósa, A.; Pavo, Noemi; Hemetsberger, Rayyan; Kvakan, Heda; Steiner-Böker, Sabine; Petrási, Zsolt; Manczur, Ferenc; Pavo, Imre J.; Édes, I.; Wojta, Johann; Glogar, Dietmar; Huber, Kurt.

In: Thrombosis and Haemostasis, Vol. 104, No. 2, 08.2010, p. 376-384.

Research output: Contribution to journalArticle

Gyöngyösi, M, Pósa, A, Pavo, N, Hemetsberger, R, Kvakan, H, Steiner-Böker, S, Petrási, Z, Manczur, F, Pavo, IJ, Édes, I, Wojta, J, Glogar, D & Huber, K 2010, 'Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion', Thrombosis and Haemostasis, vol. 104, no. 2, pp. 376-384. https://doi.org/10.1160/TH09-08-0558
Gyöngyösi, Mariann ; Pósa, A. ; Pavo, Noemi ; Hemetsberger, Rayyan ; Kvakan, Heda ; Steiner-Böker, Sabine ; Petrási, Zsolt ; Manczur, Ferenc ; Pavo, Imre J. ; Édes, I. ; Wojta, Johann ; Glogar, Dietmar ; Huber, Kurt. / Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion. In: Thrombosis and Haemostasis. 2010 ; Vol. 104, No. 2. pp. 376-384.
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AU - Hemetsberger, Rayyan

AU - Kvakan, Heda

AU - Steiner-Böker, Sabine

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