Differential distribution of NCX1 contributes to spine-dendrite compartmentalization in CA1 pyramidal cells

Andrea Lörincz, Balázs Rózsa, Gergely Katona, E. Sylvester Vizi, Gábor Tamás

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Abstract

Compartmentalization of Ca2+ between dendritic spines and shafts is governed by diffusion barriers and a range of Ca2+ extrusion mechanisms. The distinct contribution of different Ca2+ clearance systems to Ca2+ compartmentalization in dendritic spines versus shafts remains elusive. We applied a combination of ultrastructural and functional imaging methods to assess the subcellular distribution and role of NCX1 in rat CA1 pyramidal cells. Quantitative electron microscopic analysis of preembedding immunogold reactions revealed uniform densities of NCX1 along the shafts of apical and basal dendrites, but densities in dendritic shafts were approximately seven times higher than in dendritic spines. In line with these results, two-photon imaging of synaptically activated Ca2+ transients during NCX blockade showed preferential action local-zed to the dendritic shafts for NCXs in regulating spine-dendrite coupling.

Original languageEnglish
Pages (from-to)1033-1038
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number3
DOIs
Publication statusPublished - Jan 16 2007

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Keywords

  • Hippocampus
  • Imaging
  • ImmunoGold
  • Sodium-calcium exchange

ASJC Scopus subject areas

  • General

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