Differential distribution of calpain small subunit 1 and 2 in rat brain

P. Friedrich, Henrietta Papp, K. Halasy, Attila Farkas, Bence Farkas, Peter Tompa, P. Kása

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Calpains, the Ca2+-dependent thiol proteases, are abundant in the nervous tissue. The ubiquitous enzyme forms in mammals are heterodimers consisting of a specific, μ or m, large (catalytic) subunit and, apparently, a common small (regulatory) subunit (CSS1). Recently, however, we described a second form of small subunit (CSS2), which is of restricted occurrence [Schád, E., Farkas, A., Jékely, G., Tompa, P. & Friedrich, P. (2002) Biochem. J., 362, 383-388]. Here we analysed the distribution of immunoreactivity in various parts of rat brain against two anti-CSS1 and two anti-CSS2 antibodies by correlated light and electron microscopy. Remarkably, the antibodies showed differential distribution in various parts of rat cortex: anti-CSS1 reacted mainly with perikarya and dendrites, whereas anti-CSS2 was more prominent in axons. In serial sections CSS2 and synaptophysin gave very similar patterns, i.e. these epitopes seem to colocalize. Electron microscopy confirmed that CSS1 was mainly localized postsynaptically in dendrites and somata, whereas CSS2 was found presynaptically. The hypothesis is advanced that these distinct distributions of calpain subunits may be related to the transport of these enzymes in nerve cells.

Original languageEnglish
Pages (from-to)1819-1825
Number of pages7
JournalEuropean Journal of Neuroscience
Volume19
Issue number7
DOIs
Publication statusPublished - Apr 2004

Fingerprint

Calpain
Dendrites
Electron Microscopy
Nerve Tissue
Synaptophysin
Brain
Carisoprodol
Enzymes
Sulfhydryl Compounds
Axons
Epitopes
Mammals
Anti-Idiotypic Antibodies
Catalytic Domain
Peptide Hydrolases
Neurons
Light
Antibodies

Keywords

  • Anticalpain antibodies
  • Axon
  • Cortex
  • Electron microscopy
  • Immunohistochemistry
  • Perikaryon

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential distribution of calpain small subunit 1 and 2 in rat brain. / Friedrich, P.; Papp, Henrietta; Halasy, K.; Farkas, Attila; Farkas, Bence; Tompa, Peter; Kása, P.

In: European Journal of Neuroscience, Vol. 19, No. 7, 04.2004, p. 1819-1825.

Research output: Contribution to journalArticle

Friedrich, P. ; Papp, Henrietta ; Halasy, K. ; Farkas, Attila ; Farkas, Bence ; Tompa, Peter ; Kása, P. / Differential distribution of calpain small subunit 1 and 2 in rat brain. In: European Journal of Neuroscience. 2004 ; Vol. 19, No. 7. pp. 1819-1825.
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